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Cat. No.: HY-171033

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ASM-IN-3

ASM-IN-3 (Compound 21b) is a selective and BBB-penetrating acid sphingomyelinase (ASM) inhibitor (IC₅₀: 3.37 μM for pure human ASM). ASM-IN-3 improves depression-like behaviors by inhibiting ASM activity and increasing neurogenesis in hippocampus in Reserpine (HY-N0480)-induced depressed rats.

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CAS No.: 2342576-75-2
Formule: C₁₇H₁₃ClN₂O₄
Masse moléculaire:344.75

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

ASM-IN-3 Chemical Structure

CAS No.: 2342576-75-2

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
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Activité biologique

Description

Voir tous les produits spécifiques à Isoform Phospholipase :

Cellular Effect

Cell Line	Type	Value	Description	References
NIH3T3	IC₅₀	161 μM Compound: 21b	Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 31944697]

In Vitro

ASM-IN-3 (40 μM, 0.5-4 h) effectively penetrates the membrane of hCMEC/D3 cells^[1].
ASM-IN-3 (0.098-12.5 μM) is safe to NIH3T3 cells (IC₅₀: 161 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ASM-IN-3 (48 mg/kg, i.p.) penetrates the blood−brain barrier very well in SD rats, as its content in brain tissue is much higher than that in plasma^[1].
ASM-IN-3 (6-24 mg/kg, i.p., twice a day for 2 weeks) shows favorable antidepressant-like effects by decreasing brain ASM activity and restoring neurogenesis in hippocampus in Reserpine (HY-N0480)-induced depressed rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Reserpine-induced depressed SD rats (6−8 weeks, 200 g, female, were injected intraperitoneally with Reserpine (4 mg/kg) daily)^[1]
Dosage:

6, 12, 24 mg/kg
Administration:

Intraperitoneal injection (i.p.), twice a day for 2 weeks
Result:

Improved sugar consumption in a dose dependent manner after 2 days of tap water drinking and following 20 h of water depletion and fasting.
Decreased the total time in center of rats, and increased times passing the grid and frequency of grooming in the open-field test.
Demonstrated a reduction of the cortical and hippocampal ASM activity by approximately 27% and 33%, respectively, compared to the model group at a dose of 24 mg/kg.
Prevented the inhibitory effect of Reserpine on neurogenesis and restored neurogenesis.

Chemical Information

CAS No. 2342576-75-2
Masse moléculaire 344.75
Formule C₁₇H₁₃ClN₂O₄
SMILES

O=C(C1=CC(OCC2=CC=C(C3=CC=C(Cl)C=C3)C=C2)=NO1)NO
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Yang K, et al.. Discovery of Potent, Selective, and Direct Acid Sphingomyelinase Inhibitors with Antidepressant Activity. J Med Chem. 2020 Feb 13;63(3):961-974. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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