Basic green 4

Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Basic Green 4 Measurement of Phosphate^[1]
(1) Preparation of Basic Green 4 storage solution: Slowly add concentrated sulfuric acid (60 mL, d=1.84 g/L) to 300 mL of water. Cool the solution to room temperature and add 0.44 g of Basic Green 4.
(2) Preparation of Basic Green 4 working solution: Add 10 mL of the dye to 2.5 mL of 7.5% ammonium molybdate, then add 0.2 mL of 11% Tween 20.
(3) Mix 1 volume of Basic Green 4 with 4 volumes of the phosphate sample.
(4) Let it sit for ten minutes, then measure the absorbance at 630 nm.
Basic Green 4 Fungal Inhibition^[2]
(1) Collect fish eggs using spawning mats in a pond environment and rinse off the mud with well water.
(2) Place the fish eggs in a petri dish and add Basic Green 4 at a concentration of 2 ppm for 60 minutes, or at a concentration of 10 ppm for 10 minutes.
(3) Discard the Basic Green 4 and rinse the fish eggs multiple times with well water.
(4) Place the fish eggs in a petri dish with clean, oxygenated water and incubate at 22°C (72°F). Observe the newly hatched fry for 24 hours to determine the survival rate after treatment. Each concentration test is repeated six times.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.