CDy11
CDy11 is a fluorescent probe and amyloid-binding dye (λex=590 nm; λem=612 nm), with a Ka of 29 μM for Pseudomonas aeruginosa Fap. CDy11 specifically recognizes amyloid fibrils in bacterial biofilms and exhibits significantly enhanced fluorescence upon binding to the target. CDy11 shows no staining effect on amyloid-deficient mutant strains, planktonic cells or protein monomers. CDy11 supports in vivo imaging of Pseudomonas aeruginosa biofilms in mouse implant and corneal infection models. CDy11 is widely used in studies of Staphylococcus aureus biofilm infections, dental caries, and Pseudomonas aeruginosa-associated implant and corneal infections.
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- CAS No.: 2028316-07-4
- Formule: C27H23BF3N3O2
- Masse moléculaire:489.30
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
CDy11 (100 μM; 45 minutes) specifically detects amyloid fibril matrix components of Staphylococcus aureus GFP-SH1000 biofilm-coated silica microbeads, producing a strong red fluorescent signal that co-localizes with bacterial green fluorescence[1].
CDy11 detects Pseudomonas aeruginosa biofilms with a limit of 8×108 CFU/mL[2].
CDy11 (1 μM; 1 h) co-localizes with Fap-specific antibody staining in Pseudomonas aeruginosa PAO1 biofilms, confirming specific binding to Fap amyloid[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Black mice with P. aeruginosa corneal infection[2]
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Dosage:5 μM
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Administration:Topical drop; single dose; 10-minute incubation followed by PBS wash
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Result:Detected clear fluorescence signals in P.
aeruginosa-infected corneas, while no obvious signal was detected in control scratched eyes treated only with PBS.
Peaked in fluorescence intensity 10 minutes post-incubation, with most signal eliminated within 1 hour.
Showed signals colocalized with GFP signals from P.
aeruginosa and Cy5 signals from Fap (functional amyloid in Pseudomonas) antibody in immunohistochemical analysis of extracted eye sections.
Chemical Information
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CAS No. 2028316-07-4
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Masse moléculaire 489.30
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Formule C27H23BF3N3O2
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SMILES
CC(C=C1/C=C/C2=C(F)C=C(OC)C=C2)=C(N1[B-]3(F)F)C(C4=CC=C(NC(C)=O)C=C4)=C5[N+]3=CC=C5
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Sauvat L, et al. Biofilm-coated microbeads and the mouse ear skin: An innovative model for analysing anti-biofilm immune response in vivo. PLoS One. 2020;15(12):e0243500. Published 2020 Dec 4. [Content Brief]
[2]. Kim JY, et al. Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11. J Am Chem Soc. 2016;138(1):402-407. [Content Brief]
[3]. Barran-Berdon AL, et al. Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids. Sci Rep. 2020;10(1):5138. Published 2020 Mar 20. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CDy11 Related Classifications
HY-D3192 Related Classifications
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