Chlorfluazuron
Based on 1 Customer Validation
Chlorfluazuron (IKI-7899) is a benzoylurea chitin synthesis inhibitor and a PPARγ agonist ligand that blocks the polymerization of UDP-N-acetylglucosamine by inhibiting chitin synthase, disrupting cuticle formation, molting, and embryonic development, thereby exerting ovicidal, larvicidal, and fecundity-reducing effects in insects. Chlorfluazuron is used in hepatotoxicity research.
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- Pureté : 98.89%
- CAS No.: 71422-67-8
- Formule: C20H9Cl3F5N3O3
- Masse moléculaire:540.65
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Chlorfluazuron (IKI-7899) (0.166 ppm) treatment of Spodoptera littoralis 4th instar larvae results in histological abnormalities in female ovaries, including shrunken oocytes with peripheral spaces, semi-absorbed oocytes, and thin and slightly vacuolated follicular epithelium[2].
Chlorfluazuron (0.166 ppm; 48 h) treatment of Spodoptera littoralis 4th instar larvae decreases total protein (23.6 mg/g), carbohydrate (46.2 mg/g), and lipid (88.3 mg/g) contents in the ovarioles[2].
Chlorfluazuron (72 h) is highly toxic to 4th-instar Bradysia odoriphaga larvae, with an LC50 of 0.1593 mg/L at 72 h[3].
Chlorfluazuron (0.0255-0.1593 mg/L) suppresses Bradysia odoriphaga population growth under sublethal LC50 treatment by reducing the intrinsic rate of increase and finite rate of increase, net reproductive rate, survival rate, and fecundity, whereas sublethal LC10 treatment stimulates population growth by enhancing fecundity and shortening developmental time[3].
Chlorfluazuron (0.0255-0.1593 mg/L; administered daily from day 11 to day 16 after oviposition) inhibits the feeding of last-instar larvae of Bradysia odoriphaga in the short term, and this inhibitory effect is temporary and reversible[3].
Chlorfluazuron (0.0255-0.1593 mg/L; 24-72 h) initially decreases total protein levels at LC10 and LC50 concentrations but subsequently increases them, and significantly enhances GST and MFO activities in 4th-instar larvae of Bradysia odoriphaga after 48 and 72 h of treatment[3].
Chlorfluazuron exhibits high activity against third-instar larvae of Choristoneura fumiferana, first-instar larvae of Platynota stultana, and first- to second-instar larvae of Spodoptera exigua, with LC50 values of 0.27, 1.36, and 0.23 ppm, respectively[4].
Chlorfluazuron (25-0.3906 ppm; 6 days) is highly toxic to newly hatched Pectinophora gossypiella larvae, with an LC50 of 17.47 ppm and an LC90 of 253.360 ppm[5].
Chlorfluazuron (17.47 ppm; 10 days) significantly decreases total soluble protein and increases total lipid in 10-day-old Pectinophora gossypiella larvae, and increases the activities of invertase, trehalase, and amylase in 10-day-old Pectinophora gossypiella larvae[5].
Chlorfluazuron (17.47 ppm; 10 days) decreases AST activity and increases ALT activity in 10-day-old Pectinophora gossypiella larvae[5].
Chlorfluazuron (17.47 ppm; 10 days) causes histopathological alterations in the cuticle of 10-day-old Pectinophora gossypiella larvae, including thickening of the epicuticle and thinning of the endocuticle[5].
Chlorfluazuron (17.47 ppm; 10 days) causes ultrastructural alterations in the cuticle of 10-day-old Pectinophora gossypiella larvae, including necrotic lesions, separation of the cuticle from the epidermis, and damage to epidermal cells[5].
Chlorfluazuron (500-2500 ppm; 7 days) is toxic to Eobania vermiculata through contact, with an LC50 of 1528.0 ppm after 7 days[7].
Chlorfluazuron (500-2500 ppm; 7 days) is toxic to Eobania vermiculata via bait, with an LC50 of 1992.5 ppm after 7 days, but its toxicity is lower than that of the contact technique[7].
Chlorfluazuron (LC50 value; 7 days) causes severe malformations in the reproductive system of Eobania vermiculata, including swelling of the penis, vagina, and albumen gland, as well as loss of eggs from the ovotestis[7].
Chlorfluazuron (10 mg/L; 2 h) degrades under visible light irradiation in the presence of a C3N4 nanofilm, with a half-life of 1.73 h[8].
Chlorfluazuron (0.2-100 mg/L; 72 h) concentration-dependently affects the growth of Scenedesmus obliquus, with 5.0 mg/L resulting in cell viability exceeding 70%[8].
Chlorfluazuron (0.5-5.0 mg/L; 72 h) exposure significantly alters chlorophyll content in Scenedesmus obliquus in a concentration-dependent manner[8].
Chlorfluazuron (1 nM-10 μM; 24 h) acts as a PPARγ agonist in HepG2 cells, with an REC20 of 4.81 × 10-7 M and an RLA of 27.2%, exhibiting the second-highest relative agonistic potency among the five active CSIs[6].
Chlorfluazuron (below 10 μM; 24 h) does not induce significant cytotoxicity in HepG2 cells[6].
Chlorfluazuron (0.5-5.0 mg/L; 72 h) exposure induces severe oxidative stress in Scenedesmus obliquus, leading to increased Superoxide dismutase, Porcine erythrocytes (SOD) (HY-129064) activity and decreased CAT content within 72 h[8].
Chlorfluazuron (28.75-460 mg/L; mycelial growth for 14 days, conidial germination for 48 h) maintains high compatibility with Metarhizium rileyi, and does not inhibit mycelial growth, sporulation, or conidial germination within the tested dose range[9].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2
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Concentration:Below 10 μM
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Incubation Time:24 h
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Result:Did not affect the viability of HepG2 cells at concentrations below 10 μM.
In Vivo
Chlorfluazuron (5 mg/kg; seed dressing; single administration; seeds stored for 0-5 months) causes high mortality in C. chinensis adults (60.0-95.7% within 5 months) and a significant reduction in offspring (60.0-84.2%), and provides strong protection for cowpea seeds during a 5-month storage period (weight loss 4.7-11.0%)[1].
Chlorfluazuron (0.166 ppm; dietary exposure via treated castor leaves) is the most toxic IGR against S. littoralis 4th instar larvae, with an LC50 of 0.166 ppm, and it significantly reduces adult emergence, fecundity, fertility, and ovarian protein, carbohydrate, and lipid contents, while causing ovarian histological abnormalities[2].
Chlorfluazuron (25-0.3906 ppm; diet feeding; 1 hr) is highly toxic to neonate P. gossypiella larvae (LC50 = 17.47 ppm) and induces pronounced biochemical disturbances (decreased protein, increased lipid, elevated carbohydrate hydrolases and ALT, decreased AST), accompanied by severe cuticular histopathological and ultrastructural damage[5].
Chlorfluazuron (500-2500 ppm; topical film contact exposure; single exposure; 7 days) is effective against Eobania vermiculata, with LC50 values of 1528.0 ppm (contact) and 1992.5 ppm (bait) after 7 days of treatment[7].
Chlorfluazuron (1528.0-1992.5 ppm; topical film contact exposure; single exposure; 7 days) at its LC50 concentration induces severe malformations of the reproductive system and causes cessation of egg-laying in Eobania vermiculata[7].
Chlorfluazuron (50.0 mL/L; spray application; single treatment; 7 days) achieves 78.7% population reduction of Eobania vermiculata under field conditions[7].
Chlorfluazuron (IKI-7899) (17.25-276.00 mg/L; dip treatment; 10 s) exhibits strong larvicidal activity against third-instar S. frugiperda, with a 6-day LC50 of 13.70 mg/L[9].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Callosobruchus maculatus (adult, 1-2 days old, mixed sex)[1]
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Dosage:5.0 mg/kg
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Administration:admixture; single dose; seeds stored for 0-5 months
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Result:Achieved adult mortality of 98.5% at month 0, 61.8% at month 1, 51.5% at month 2, 82.9% at month 3, 52.8% at month 4, and 74.5% at month 5.
Reduced progeny production by 73.2% at month 0, 78.4% at month 1, 53.4% at month 2, 81.6% at month 3, 76.8% at month 4, and 38.1% at month 5.
Resulted in cowpea seed weight loss of 8.3% at month 0, 7.5% at month 1, 24.5% at month 2, 4.4% at month 3, 7.0% at month 4, and 17.6% at month 5.
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Animal Model:Callosobruchus chinensis (adult, 1-2 days old, mixed sex)[1]
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Dosage:5.0 mg/kg
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Administration:admixture; single dose; seeds stored for 0-5 months
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Result:Achieved adult mortality of 95.7% at month 0, 74.9% at month 1, 60.0% at month 2, 88.4% at month 3, 73.6% at month 4, and 82.8% at month 5.
Reduced progeny production by 60.0% at month 0, 74.9% at month 1, 60.9% at month 2, 84.2% at month 3, 73.5% at month 4, and 67.9% at month 5.
Resulted in cowpea seed weight loss of 9.8% at month 0, 5.9% at month 1, 11.0% at month 2, 4.7% at month 3, 8.1% at month 4, and 10.4% at month 5.
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Animal Model:Spodoptera littoralis (Boisd.) (4th instar larvae)[2]
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Dosage:0.166 ppm
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Administration:dietary exposure via treated castor bean leaves
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Result:Reduced adult emergence by 16% compared to control (84% emergence).
Reduced fecundity to 151 eggs per female in T♀ × T♂, 170 eggs per female in T♀ × N♂, and 180 eggs per female in N♀ × T♂ (control = 408 eggs per female).
Reduced egg hatchability to 65.0% in T♀ × T♂, 66.0% in T♀ × N♂, and 71.0% in N♀ × T♂ (control = 91.8%).
Decreased total protein content in ovaries to 23.6 mg/g fresh tissue.
Decreased total carbohydrate content in ovaries to 46.2 mg/g fresh tissue.
Decreased total lipid content in ovaries to 88.3 mg/g fresh tissue.
Caused histological abnormalities in the ovary including oocyte shrinkage, semi-absorbed oocytes, and thin vacuolated follicular epithelium.
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Animal Model:Bradysia odoriphaga Yang and Zhang (Diptera: Sciaridae) (fourth instar larvae, 26 °C, 65-75% RH, 12:12 h L:D)[3]
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Dosage:0.0255 mg/L (LC10); 0.1593 mg/L (LC50)
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Administration:topical; contact/ingestion
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Result:Achieved a 72 h LC50 of 0.1593 mg/L (95% CI: 0.1195-0.2136) against fourth instar larvae.
Shortened larval development duration to 13.60 days vs. 14.33 days control at LC50.
Shortened male adult longevity to 3.29 days vs. 3.74 days control at LC50.
Increased fecundity to 134.7 eggs per female vs. 123.1 control at LC10.
Decreased the intrinsic rate of increase to 0.1447 d-1 vs. 0.1684 d-1 control at LC50.
Decreased the net reproductive rate to 25.97 vs. 52.91 offspring/individual control at LC50.
Decreased the mean generation time to 22.44 days vs. 23.54 days control at LC50.
Increased the intrinsic rate of increase to 0.1713 d-1 at LC10.
Inhibited feeding of final instar larvae for a short period, with feeding amounts declining significantly on the treatment day and returning to control levels within two days.
Caused pupal blackening, head and abdomen distention, wing dysplasia, and failure of adult emergence at LC50.
Decreased total protein from 24 to 48 h and increased it from 48 to 72 h in LC10 and LC50 treatments.
Increased GST and MFO activities at 48 and 72 h after LC10 and LC50 treatments compared with controls.
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Animal Model:Eobania vermiculata (hermaphrodite; collected from citrus nursery trees, Ministry of Agriculture, Giza Governorate)[7]
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Dosage:500, 1000, 1500, 2000, 2500 ppm
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Administration:topical contact exposure to thin film; single exposure; 7 days (contact technique); p.o.; single exposure via poison bait; 7 days (bait technique)
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Result:Caused 10%, 20%, 40%, 60%, and 90% mortality at 500, 1000, 1500, 2000, and 2500 ppm, respectively, with an LC50 of 1528.0 ppm (slope 0.52) in the contact technique.
Caused 10%, 10%, 30%, 50%, and 70% mortality at 500, 1000, 1500, 2000, and 2500 ppm, respectively, with an LC50 of 1992.5 ppm (slope 0.59) in the bait technique.
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Animal Model:Eobania vermiculata (hermaphrodite)[7]
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Dosage:1528.0 ppm (contact technique); 1992.5 ppm (bait technique)
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Administration:topical contact exposure to thin film; single exposure; 7 days (contact technique); p.o.; single exposure via poison bait; 7 days (bait technique)
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Result:Induced malformation in the reproductive system organs compared with untreated animals.
Caused severe swelling in the size of the penis, vagina, sperm oviduct, and albumen gland, and stunting in the sperm oviduct and albumen gland length.
Tumor occurred in the albumen gland.
In the ovotestis, eggs disappeared from treated snails, whereas eggs were present inside the ovotestis of untreated snails.
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Animal Model:Eobania vermiculata (hermaphrodite)[7]
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Dosage:50.0 mL/L
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Administration:spray application; single treatment; 7-day observation
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Result:Caused a 78.7% reduction in snail population after 7 days (89 animals before treatment, 32 live animals after treatment).
Chemical Information
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CAS No. 71422-67-8
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Appearance Solid
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Masse moléculaire 540.65
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Formule C20H9Cl3F5N3O3
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Color White to off-white
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SMILES
O=C(NC(NC1=CC(Cl)=C(OC2=NC=C(C(F)(F)F)C=C2Cl)C(Cl)=C1)=O)C3=C(F)C=CC=C3F
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Synonyms
IKI-7899
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 250 mg/mL (462.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (300 KB)
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SDS (536 KB)
- English - EN (536 KB)
- Français - FR (536 KB)
- Deutsch - DE (536 KB)
- Norwegian - NO (536 KB)
- Español - ES (536 KB)
- Swedish - SV (536 KB)
- Italian - IT (536 KB)
- Korean - KR (536 KB)
- Portuguese - PT (536 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8496 mL | 9.2481 mL | 18.4963 mL | 46.2406 mL |
| 5 mM | 0.3699 mL | 1.8496 mL | 3.6993 mL | 9.2481 mL | |
| 10 mM | 0.1850 mL | 0.9248 mL | 1.8496 mL | 4.6241 mL | |
| 15 mM | 0.1233 mL | 0.6165 mL | 1.2331 mL | 3.0827 mL | |
| 20 mM | 0.0925 mL | 0.4624 mL | 0.9248 mL | 2.3120 mL | |
| 25 mM | 0.0740 mL | 0.3699 mL | 0.7399 mL | 1.8496 mL | |
| 30 mM | 0.0617 mL | 0.3083 mL | 0.6165 mL | 1.5414 mL | |
| 40 mM | 0.0462 mL | 0.2312 mL | 0.4624 mL | 1.1560 mL | |
| 50 mM | 0.0370 mL | 0.1850 mL | 0.3699 mL | 0.9248 mL | |
| 60 mM | 0.0308 mL | 0.1541 mL | 0.3083 mL | 0.7707 mL | |
| 80 mM | 0.0231 mL | 0.1156 mL | 0.2312 mL | 0.5780 mL | |
| 100 mM | 0.0185 mL | 0.0925 mL | 0.1850 mL | 0.4624 mL |
Chlorfluazuron Related Classifications
HY-133204 Related Classifications
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