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Cat. No.: HY-D3255

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CTAP-1

CTAP-1 is a monovalent copper ion probe. CTAP-1 binds to monovalent copper Cu (I) to inhibit photoinduced electron transfer, thereby enhancing fluorescence emission. CTAP-1 produces copper-dependent perinuclear staining that colocalizes with mitochondria and the Golgi apparatus. CTAP-1 can be used for imaging Cu (I) pools, excitation/emission Wavelength: 365/480 nm.

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CAS No.: 866534-64-7
Formule: C₃₂H₃₇N₃O₂S₄
Masse moléculaire:623.92

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Please store the product under the recommended conditions in the Certificate of Analysis.

CTAP-1 Chemical Structure

CAS No.: 866534-64-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
Chemical Information
Pureté et documentation
Références
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Activité biologique

Description

In Vitro

CTAP-1 (10 μM; 50 min incubation) labels a perinuclear, copper-dependent labile copper pool in NIH 3T3 fibroblast cells, with fluorescence intensity increasing ~2- to 3-fold in copper-supplemented medium, and staining colocalizing with mitochondria and the Golgi apparatus^[1].
CTAP-1 (2 μM) fluorescence in copper-loaded NIH 3T3 fibroblast cells is reduced by ~50% upon treatment with the exogenous Cu⁺ chelator DTO, confirming the signal is copper-dependent^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

mouse NIH 3T3 fibroblast cells
Concentration:

10 μM (standard staining; immunofluorescence colocalization); 150 μM CuCl₂ (cell pre-culture)
Incubation Time:

50 min (37 °C, DMEM for standard staining; room temperature, PBS for immunofluorescence colocalization); 12 h (cell pre-culture with CuCl₂)
Result:

Produced a weak perinuclear staining pattern in basal medium.
Showed an ~2- to 3-fold stronger emission intensity in cells pre-cultured with 150 μM CuCl₂ for 12 h.
Colocalized staining pattern with subcellular locations of the Golgi apparatus and mitochondria.

Chemical Information

CAS No. 866534-64-7
Masse moléculaire 623.92
Formule C₃₂H₃₇N₃O₂S₄
SMILES

[H][C@]1(CC(C2=CC=CC=C2)=NN1C3=CC=C(C(O)=O)C=C3)C(C=C4)=CC=C4N5CCSCCSCCSCCSCC5
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Yang L, et al. Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy. Proc Natl Acad Sci U S A. 2005;102(32):11179-11184. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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