Cat. No.: HY-Y1366

Hydroxyacetone

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Hydroxyacetone is a toxic compound. Hydroxyacetone can be isolated from e-cigarette aerosols. Hydroxyacetone reduces the activity of cellular Mitochondrial reductase (with an IC₅₀ of 5.53 mg/mL for mitochondrial reductase in BEAS-2B cells) and increases ROS levels. Hydroxyacetone induces mitochondrial stress and oxidative damage. Hydroxyacetone induces destabilization of F-actin. At high concentrations, Hydroxyacetone promotes cell rounding and Apoptotic body formation. Hydroxyacetone exerts toxic effects on cells including airway epithelial cells and possesses respiratory toxicity potential.\n

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Pureté: 98.93%
CAS No.: 116-09-6
Formule: C₃H₆O₂
Masse moléculaire:74.08

Stockage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Hydroxyacetone Chemical Structure

CAS No.: 116-09-6

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Hydroxyacetone (0.01-10 mg/mL; 24 h) reduces mitochondrial reductase activity in BEAS-2B cells with an IC₅₀ of 5.53 mg/mL and an IC₇₀ of 2.49 mg/mL after 24 hours of exposure^[1].
Hydroxyacetone (0.01-10 mg/mL; 24 h) increases mitochondrial membrane potential in BEAS-2B cells at 1 mg/mL and reduces it at 10 mg/mL after 24 hours of exposure^[1].
Hydroxyacetone (0.01-10 mg/mL; 24 h) increases mitochondrial superoxide production in BEAS-2B cells at 1 mg/mL, with cell rounding and concentrated fluorescence observed at 10 mg/mL after 24 hours of exposure^[1].
Hydroxyacetone (0.01-1 mg/mL; 24 h) significantly increases hydrogen peroxide release into the culture medium of BEAS-2B cells after 24 hours of incubation^[1].
Hydroxyacetone (1-10 mg/mL; 2-8 h) induces concentration- and time-dependent F-actin destabilization in BEAS-2B cells, with cytoplasmic and perinuclear filaments more sensitive than cortical actin, and complete loss of filamentous actin with cell rounding and blebbing at 10 mg/mL after 8 hours of exposure^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

BEAS-2B human bronchial epithelial cells
Concentration:

0.01-10 mg/mL
Incubation Time:

24 h
Result:

Showed concentration-dependent reduction in mitochondrial reductase activity.
Determined IC₅₀ (concentration reducing activity by 50%) as 5.53 mg/mL, and IC₇₀ (concentration reducing activity by 70%) as 2.49 mg/mL.
Observed a significant reduction in activity at 10 mg/mL.

Chemical Information

CAS No. 116-09-6
Appearance Liquid (Density: 1.082 g/cm³)
Masse moléculaire 74.08
Formule C₃H₆O₂
Color Colorless to light yellow
SMILES

CC(CO)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (1349.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

13.4989 mL

67.4946 mL

134.9892 mL

5 mM

2.6998 mL

13.4989 mL

26.9978 mL

10 mM

1.3499 mL

6.7495 mL

13.4989 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (33.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (33.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (33.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (272 KB)
SDS (466 KB)
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- Italian - IT (466 KB)
- Korean - KR (466 KB)
- Portuguese - PT (466 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Wong M, et al. Unraveling the Toxicological Effects of Hydroxyacetone - A Reaction Product in Electronic Cigarette Aerosols. bioRxiv [Preprint]. 2025 Sep 4:2025.08.29.673133. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	13.4989 mL	67.4946 mL	134.9892 mL	337.4730 mL
	5 mM	2.6998 mL	13.4989 mL	26.9978 mL	67.4946 mL
	10 mM	1.3499 mL	6.7495 mL	13.4989 mL	33.7473 mL
	15 mM	0.8999 mL	4.4996 mL	8.9993 mL	22.4982 mL
	20 mM	0.6749 mL	3.3747 mL	6.7495 mL	16.8736 mL
	25 mM	0.5400 mL	2.6998 mL	5.3996 mL	13.4989 mL
	30 mM	0.4500 mL	2.2498 mL	4.4996 mL	11.2491 mL
	40 mM	0.3375 mL	1.6874 mL	3.3747 mL	8.4368 mL
	50 mM	0.2700 mL	1.3499 mL	2.6998 mL	6.7495 mL
	60 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	80 mM	0.1687 mL	0.8437 mL	1.6874 mL	4.2184 mL
	100 mM	0.1350 mL	0.6749 mL	1.3499 mL	3.3747 mL

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Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

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