PDDC
Based on 1 Customer Validation
PDDC is an orally active and blood-brain barrier-penetrant nSMase2 inhibitor. PDDC reduces ceramide production and extracellular vesicle biogenesis/release through enzyme inhibition (IC50 300 nM). PDDC reduces IL-1β-induced release of plasma extracellular vesicles from neurons, oligodendrocytes, and activated microglia, and decreases the ratio of CD11b-positive/IB4-positive extracellular vesicles. PDDC can be used in research related to acute brain injury.
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- Pureté : 99.58%
- CAS No.: 2232878-43-0
- Formule: C27H29N5O4
- Masse moléculaire:487.55
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
0.3 μM
Compound: 1; PDDC
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Inhibition of human recombinant full-length nSMase expressed in HEK293 cells using sphingomyelin as substrate measured after 1 hr by alkaline phosphatase, choline oxidase and horseradish peroxidase dependent H2O2 detection-coupled amplex reagent based flu
Inhibition of human recombinant full-length nSMase expressed in HEK293 cells using sphingomyelin as substrate measured after 1 hr by alkaline phosphatase, choline oxidase and horseradish peroxidase dependent H2O2 detection-coupled amplex reagent based flu
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[PMID: 32298582] |
Parmacokinetics
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:GFAP-GFP transgenic (male; 2-3 months old; Jackson Strain #003257)[1]
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Dosage:30 mg/kg; 100 mg/kg
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Administration:p.o.; daily; initiated 6 days before intra-striatal injection
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Result:Normalized striatal nSMase2 activity and reduces plasma total, neuronal, oligodendroglial, and activated microglial EV populations while not changing astrocyte-derived EVs in male GFAP-GFP mice with intra-striatal IL-1β injury.
Chemical Information
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CAS No. 2232878-43-0
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Appearance Solid
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Masse moléculaire 487.55
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Formule C27H29N5O4
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Color White to off-white
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SMILES
CC1=C(C2=CC(OC)=C(C=C2)OC)N(N=C(C=C3N4C[C@@H](CC4)NC(OC5=CC=CC=C5)=O)C)C3=N1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (205.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0511 mL | 10.2554 mL | 20.5107 mL | 51.2768 mL |
| 5 mM | 0.4102 mL | 2.0511 mL | 4.1021 mL | 10.2554 mL | |
| 10 mM | 0.2051 mL | 1.0255 mL | 2.0511 mL | 5.1277 mL | |
| 15 mM | 0.1367 mL | 0.6837 mL | 1.3674 mL | 3.4185 mL | |
| 20 mM | 0.1026 mL | 0.5128 mL | 1.0255 mL | 2.5638 mL | |
| 25 mM | 0.0820 mL | 0.4102 mL | 0.8204 mL | 2.0511 mL | |
| 30 mM | 0.0684 mL | 0.3418 mL | 0.6837 mL | 1.7092 mL | |
| 40 mM | 0.0513 mL | 0.2564 mL | 0.5128 mL | 1.2819 mL | |
| 50 mM | 0.0410 mL | 0.2051 mL | 0.4102 mL | 1.0255 mL | |
| 60 mM | 0.0342 mL | 0.1709 mL | 0.3418 mL | 0.8546 mL | |
| 80 mM | 0.0256 mL | 0.1282 mL | 0.2564 mL | 0.6410 mL | |
| 100 mM | 0.0205 mL | 0.1026 mL | 0.2051 mL | 0.5128 mL |
PDDC Related Classifications
HY-156800 Related Classifications
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