Phaseoloideside C
Phaseoloideside C is an oleanane-type triterpenoid saponin. Phaseoloideside C can be isolated from the seeds of *Entada phaseoloides*. Phaseoloideside C exhibits no cytotoxicity against hepatocellular carcinoma cells, esophageal carcinoma cells, cervical carcinoma cells, and colon carcinoma cells .
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- CAS No.: 1453187-72-8
- Formule: C70H113NO36
- Masse moléculaire:1544.63
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Phaseoloideside C (48 h) exhibits no cytotoxicity against HepG-2, Eca-109, SW480, and HeLa cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepG-2 (human hepatocellular carcinoma cell line), Eca-109 (human esophageal carcinoma cell line), SW480 (human colon carcinoma cell line), HeLa (human epithelial carcinoma cell line)
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Concentration:unspecified
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Incubation Time:48 h (incubation after compound addition)
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Result:Exhibited no in vitro cytotoxicity against HepG-2, Eca-109, SW480, and HeLa cell lines.
Chemical Information
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CAS No. 1453187-72-8
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Masse moléculaire 1544.63
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Formule C70H113NO36
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SMILES
O[C@H]([C@](O)(CO1)CO[C@H]2[C@@H]([C@](O)(CO2)CO)O)[C@@H]1O[C@H]([C@H]([C@@H]([C@H](O3)CO)O)O)[C@@H]3OC([C@]45[C@](CC(C)(CC5)C)([H])C6=CC[C@@]([C@@]7([C@@](C(C)([C@H](CC7)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO[C@H]9[C@@H]([C@H]([C@H](CO9)O)O[C@H]%10[C@@H]([C@H]([C@@H](CO%10)O)O)O)O)O[C@@H]%11O[C@@H]([C@H]([C@@H]([C@H]%11O)O)O)CO)O)NC(C)=O)C)([H])CC%12)C)([H])[C@]%12(C)[C@@]6([C@H]([C@H]4O)O)C)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phaseoloideside C Related Classifications
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