Urdamycin B
Urdamycin B is an antibiotic that effectively inhibits fungi and bacteria. Urdamycin B also exhibits anti-proliferative activity against mouse leukemia cells L1210. Urdamycin B can be obtained from the metabolic products of Streptomyces fradiae. Urdamycin B can be used for research on cancer as well as bacterial and fungal infections.
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- CAS No.: 104542-46-3
- Formule: C37H44O13
- Masse moléculaire:696.74
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
Description
Chemical Information
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CAS No. 104542-46-3
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Masse moléculaire 696.74
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Formule C37H44O13
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SMILES
O=C1C2=C(C(C3=C(O)C([C@H]4C[C@H]([C@@H]([C@H](O4)C)O)O[C@@H]5O[C@H]([C@H](CC5)O[C@H]6C[C@H]([C@@H]([C@H](O6)C)O)O)C)=CC=C31)=O)C=CC(C[C@@](O)(C7)C)=C2C7=O
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Structure Classification
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Initial Source
Streptomyces fradiae
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)