Chlorophosphonazo III

Chlorophosphonazo III (150 μM) forms a stable complex with Ca²⁺. The absorbance of this complex increases linearly in a wavelength-dependent manner with elevated Ca²⁺ concentration, which is particularly pronounced at 627 nm, rendering it suitable for photoacoustic Ca²⁺ imaging^[1].
Chlorophosphonazo III (50-5000 μM; 30 min) is cell membrane-permeable and exhibits no cytotoxicity against Neuro2A cells at concentrations up to 1000 μM^[1].
Chlorophosphonazo III (100-150 μM) generates PA signals that are linearly correlated with Ca²⁺ concentration, among which 150 μM CPZ III provides a more predictable correlation than 100 μM; an 8 MHz US transducer enables optimal detection of these PA signals to achieve accurate Ca²⁺ quantitative analysis^[1].
Chlorophosphonazo III (100-150 μM; 60 min) enables real-time, depth-resolved imaging of spatiotemporal Ca²⁺ dynamics in 3D Neuro2A tumor spheroids, revealing delayed Ca²⁺ responses and greater endoplasmic reticulum Ca²⁺ release in hypoxic core regions compared with normoxic edge regions^[1].
Chlorophosphonazo III (0.2 nM-0.2 μM; 10 min-1 week) forms stable 1:1 complexes with Ca²⁺ and Mg²⁺, enabling highly sensitive spectrophotometric determination of these metals (with a detection limit as low as 0.1 μg per 25 mL when using a 5-cm cuvette at pH 7.0) with good precision; specifically, Ca²⁺ can be selectively determined at pH 2.2, while the additivity of absorbance allows simultaneous quantification of both metals at pH 7.0^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.