CIM-834
Based on 1 Customer Validation
CIM-834 is an orally effective inhibitor of SARS-CoV-2 membrane protein. CIM-834 can prevent the assembly of infectious virus particles without inhibiting the synthesis of viral RNA. CIM-834 can reduce the viral titer in the lungs of SCID mice infected nasally with SARS-CoV-2, block the spread of SARS-CoV-2 among Syrian hamsters, and inhibit the replication of SARS-CoV-2 (including variants) and SARS-CoV. CIM-834 can be used in related research on COVID-19.
For research use only. We do not sell to patients.
- Purity: 99.14%
- CAS No.: 3104363-32-5
- Formula: C27H42N8O
- Molecular Weight:494.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
HY-L002
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Antiviral Compound Library
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Orally Active Compound Library
HY-L061
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Bioactive Compound Library Max
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
CIM-834 (0-10 μM; 3-5 d) potently inhibits SARS-CoV and HCoV-OC43 replication, exhibits partial activity against HCoV-229E, and lacks activity against HCoV-NL63, FIPV, PEDV, and MERS-CoV[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
CIM-834 (100 mg/kg, p.o., twice daily for 4 days, in combination with ritonavir 50 mg/kg, p.o., twice daily) reduces lung SARS-CoV-2 titers in Syrian hamsters[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CB-17/Icr-Prkdcscid/scid/Rj SCID mice (male, 7-9 weeks old, intranasal infection with SARS-CoV-2 B.1.351)[1]
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Dosage:100 mg/kg (once daily; twice daily)
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Administration:p.o.; once daily; 3 days; p.o.; twice daily; 3 days
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Result:Significantly and dose-dependently reduced the infectious virus titer and viral RNA load in lung tissue.
Reduced the infectious virus titer in the mouse lungs by more than 3 log₁₀ within 48 hours administration after infection.
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Animal Model:Syrian hamsters (female, 8-10 weeks old, intranasal infection with SARS-CoV-2 USA-WA1/2020)[1]
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Dosage:100 mg/kg (CIM-834); 50 mg/kg (ritonavir)
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Administration:p.o.; twice daily; 4 days (CIM-834 co-administered with ritonavir)
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Result:Reduced lung viral RNA levels by 3.4 log10 and infectious viral titres by greater than 4 log10.
Resulted in nearly normal lung parenchyma with only very focal perivascular inflammation.
Prevented transmission of SARS-CoV-2 from treated index hamsters to untreated sentinel hamsters.
Chemical Information
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CAS No. 3104363-32-5
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Appearance Solid
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Molecular Weight 494.68
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Formula C27H42N8O
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Color White to off-white
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SMILES
CC1=NC(N2CCC(N(C)C3=CC(C(NCCN4CCCC4)=O)=CN=N3)CC2)=NC(C)=C1CC(C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (202.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0215 mL | 10.1075 mL | 20.2151 mL | 50.5377 mL |
| 5 mM | 0.4043 mL | 2.0215 mL | 4.0430 mL | 10.1075 mL | |
| 10 mM | 0.2022 mL | 1.0108 mL | 2.0215 mL | 5.0538 mL | |
| 15 mM | 0.1348 mL | 0.6738 mL | 1.3477 mL | 3.3692 mL | |
| 20 mM | 0.1011 mL | 0.5054 mL | 1.0108 mL | 2.5269 mL | |
| 25 mM | 0.0809 mL | 0.4043 mL | 0.8086 mL | 2.0215 mL | |
| 30 mM | 0.0674 mL | 0.3369 mL | 0.6738 mL | 1.6846 mL | |
| 40 mM | 0.0505 mL | 0.2527 mL | 0.5054 mL | 1.2634 mL | |
| 50 mM | 0.0404 mL | 0.2022 mL | 0.4043 mL | 1.0108 mL | |
| 60 mM | 0.0337 mL | 0.1685 mL | 0.3369 mL | 0.8423 mL | |
| 80 mM | 0.0253 mL | 0.1263 mL | 0.2527 mL | 0.6317 mL | |
| 100 mM | 0.0202 mL | 0.1011 mL | 0.2022 mL | 0.5054 mL |
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