2. TGF-beta/Smad
  3. TGF-β Receptor
  4. CJJ300

CJJ300 is a transforming growth factor-β (TGF-β) inhibitor with an IC50 of 5.3 µM. CJJ300 inhibits TGF-β signaling by disrupting the formation of the TGF-β-TβR-I-TβR-II signaling complex.

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CJJ300 Chemical Structure

CJJ300 Chemical Structure

CAS No. : 1807631-83-9

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 107 In-stock
Solution
10 mM * 1 mL in DMSO USD 107 In-stock
Solid
5 mg USD 98 In-stock
10 mg USD 170 In-stock
25 mg USD 340 In-stock
50 mg USD 550 In-stock
100 mg USD 850 In-stock
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Based on 1 publication(s) in Google Scholar

CJJ300 Related Antibodies

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Description

CJJ300 is a transforming growth factor-β (TGF-β) inhibitor with an IC50 of 5.3 µM. CJJ300 inhibits TGF-β signaling by disrupting the formation of the TGF-β-TβR-I-TβR-II signaling complex[1].

IC50 & Target

IC50: 5.3 µM (TGF-β1-induced luciferase)[1]
In Vitro

CJJ300 disturbs protein-protein interactions and prevents TGF-β receptor dimerization (IC50 = 23.6 ± 5.8 µM)[1].
CJJ300 (0-80 µM, 2 h) inhibits the phosphorylation of intracellular mediators in the downstream TGF-β signaling pathways, and suppresses the expression of markers of EMT (epithelial-mesenchymal transition) without cytotoxicity[1].
CJJ300 suppresses TGF-β induced cell migration[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CJJ300 Related Antibodies

Western Blot Analysis[1]

Cell Line: Human A549 lung epithelial cells
Concentration: 20, 40, and 80 µM
Incubation Time: 2 h
Result: Significantly attenuated the increasement of P-Smad2/Smad3, P-Erk1/2 and P-Akt induced by TGF-β. Down regulated the expression of EMT-associated proteins, including fibronectin, α-SMA and MMP-2 in a dose-dependent manner.
Molecular Weight

435.60

Formula

C30H33N3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(C(C)=C(C(C)=C1CNC2=CC=CC=C2)CNC3=CC=CC=C3)CNC4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (229.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2957 mL 11.4784 mL 22.9568 mL
5 mM 0.4591 mL 2.2957 mL 4.5914 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (5.74 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.77%

COA (249 KB) HNMR (137 KB) RP-HPLC (169 KB) LCMS (357 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2957 mL 11.4784 mL 22.9568 mL 57.3921 mL
5 mM 0.4591 mL 2.2957 mL 4.5914 mL 11.4784 mL
10 mM 0.2296 mL 1.1478 mL 2.2957 mL 5.7392 mL
15 mM 0.1530 mL 0.7652 mL 1.5305 mL 3.8261 mL
20 mM 0.1148 mL 0.5739 mL 1.1478 mL 2.8696 mL
25 mM 0.0918 mL 0.4591 mL 0.9183 mL 2.2957 mL
30 mM 0.0765 mL 0.3826 mL 0.7652 mL 1.9131 mL
40 mM 0.0574 mL 0.2870 mL 0.5739 mL 1.4348 mL
50 mM 0.0459 mL 0.2296 mL 0.4591 mL 1.1478 mL
60 mM 0.0383 mL 0.1913 mL 0.3826 mL 0.9565 mL
80 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7174 mL
100 mM 0.0230 mL 0.1148 mL 0.2296 mL 0.5739 mL
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CJJ300 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CJJ3001807631-83-9CJJ 300CJJ-300TGF-β ReceptorTransforming growth factor beta receptorsA549Protein-protein interactionP-Smad2/Smad3P-Erk1/2P-AktEMTInhibitorinhibitorinhibit

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