CuFS 

CuFS is a reaction-based fluorescent sensor for detecting Cu²⁺ with zero background fluorescence. CuFS acts as a fluorescence quencher in the absence of Cu²⁺ and as a fluorescence enhancer in the presence of Cu²⁺. CuFS can be fabricated into test strips and applied to the detection of Cu²⁺ in human cancer cells^[1].

CuFS (10 μM; 30 min at room temperature) shows almost zero background fluorescence in PBS containing 20% THF. After reaction with 22 μM Cu²⁺, it produces an 811-fold fluorescence enhancement centered at 469 nm. Its detection limit for Cu²⁺ is 12.3 nM, with a linear response range from 12.3 nM to 2.0 μM^[1].
CuFS (0-20 μM; incubated at 37 °C for 30 min) exhibits negligible background fluorescence and induces concentration-dependent fluorescence enhancement in human hepatocellular carcinoma SMMC-7721 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

457.46

C₂₀H₁₉N₅O₆S

1860808-05-4

CCN(C1=CC=C2C=C(C(OC2=C1)=O)C(NNC3=CC=C(C=C3[N+]([O-])=O)[N+]([O-])=O)=S)CC

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Long L, et al. Complete suppression of the fluorophore fluorescence by combined effect of multiple fluorescence quenching groups: A fluorescent sensor for Cu²⁺ with zero background signals. Anal Chim Acta. 2016;908:1-7.