Cy5-Paclitaxel
Based on 1 Customer Validation
Cy5-Paclitaxel is a fluorescently labeled drug probe formed by conjugating the fluorescent dye Cy5 (Cyanine 5) with the chemotherapeutic agent Paclitaxel, which is used to track the cellular uptake, subcellular distribution and drug delivery process of Paclitaxel. The Cy5 (Cyanine 5) fluorophore is covalently linked to Paclitaxel, possessing both fluorescent tracing function and the biological activities of Paclitaxel (promoting tubulin polymerization and arresting the cell cycle at the G2/M phase); after entering cells, Cy5 emits far-red fluorescence, enabling real-time monitoring of drug localization and release.
For research use only. We do not sell to patients.
- Purity: 95.60%
- Formula: C85H99ClN4O16
- Molecular Weight:1468.17
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Operating Instructions
(The following is a recommended experimental protocol for guidance only; adjust according to actual requirements)
1. Stock Solution Preparation
1.1 Solvent: For most dyes, organic solvents are commonly used for dissolution, such as anhydrous DMSO.
1.2 Concentration Recommendation: It is generally recommended to prepare a high-concentration stock solution of 1-10 mM.
2. Working Solution Preparation
2.1 Diluent: Cell culture medium.
2.2 Working Concentrations: 50 nM, 100 nM, 200 nM, 400 nM.
2.3 Notes: Adjust the working solution concentration as needed; prepare and use immediately.
3. Staining Procedure
3.1 Sample Type: Adherent breast cancer cells (MDA-MB-231)
3.2 Incubation Conditions: Incubate at 37°C for 48 h or 72 h in a humidified environment containing 5% CO2.
3.3 Washing Step: No washing step is specified in this experiment.
4. Control Group Setup
4.1 Blank Control: Unconjugated RNA pyramid nanoparticles (at the same test concentration as Cy5-paclitaxel-conjugated nanoparticles).
5. Detection and Analysis
5.1 Instruments: Fluorescence imaging system for Cy5 detection; microplate reader for absorbance detection.
5.2 Result Analysis:
5.2.1 Fluorescence Intensity Change: After irradiation with 365 nm ultraviolet light, the fluorescence intensity of Cy5 decreases.
5.2.2 Cell Viability: Compared with unirradiated samples and samples without photocleavable spacers, the Cy5-paclitaxel-conjugated RNA nanocages with photocleavable spacers show enhanced inhibitory effect on cell viability after ultraviolet irradiation.
5.2.3 Color Change: Cell viability is evaluated by detecting absorbance at 570 nm via MTT assay, where a decrease in absorbance corresponds to reduced cell viability.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1468.17
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Formula C85H99ClN4O16
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Color Light blue to blue
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SMILES
O=C(NCCCCCC(O[C@@H](C(O[C@H]1C[C@@]([C@H]([C@]([C@](C([C@@H]2OC(C)=O)=O)([C@H]3O)C)([H])[C@]4([C@@H](C3)OC4)OC(C)=O)OC(C5=CC=CC=C5)=O)(C(C)(C2=C1C)C)O)=O)[C@@H](NC(C6=CC=CC=C6)=O)C7=CC=CC=C7)=O)CCCCC[N+]8=C(/C=C/C=C/C=C9C(C)(C)C(C=CC=C%10)=C%10N\9C)C(C)(C)C%11=C8C=CC=C%11.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (68.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.6811 mL | 3.4056 mL | 6.8112 mL | 17.0280 mL |
| 5 mM | 0.1362 mL | 0.6811 mL | 1.3622 mL | 3.4056 mL | |
| 10 mM | 0.0681 mL | 0.3406 mL | 0.6811 mL | 1.7028 mL | |
| 15 mM | 0.0454 mL | 0.2270 mL | 0.4541 mL | 1.1352 mL | |
| 20 mM | 0.0341 mL | 0.1703 mL | 0.3406 mL | 0.8514 mL | |
| 25 mM | 0.0272 mL | 0.1362 mL | 0.2724 mL | 0.6811 mL | |
| 30 mM | 0.0227 mL | 0.1135 mL | 0.2270 mL | 0.5676 mL | |
| 40 mM | 0.0170 mL | 0.0851 mL | 0.1703 mL | 0.4257 mL | |
| 50 mM | 0.0136 mL | 0.0681 mL | 0.1362 mL | 0.3406 mL | |
| 60 mM | 0.0114 mL | 0.0568 mL | 0.1135 mL | 0.2838 mL |
Cy5-Paclitaxel Related Classifications
HY-D2422B Related Classifications
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