Cyanine5 alkyne  (Synonyms: Alkyne-Cy5)

Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC)^[1][2]. Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Cyanine5 alkyne (Alkyne-Cy5) (1-1000 nM) significantly inhibits 3D mammosphere formation in MCF7 cells between 500 nM and 1000 nM and can act as a mitochondrial oxidative phosphorylation (OXPHOS) inhibitor, inducing glycolysis to compensate for mitochondrial ATP depletion^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

556.18

C₃₅H₄₂ClN₃O

1223357-57-0

670

645

C#CCNC(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)C(C)(C)C4=C1C=CC=C4)=O.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Amanda R Burnham-Marusich, et al. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis. 2014 Sep;35(18):2621-5.  [Content Brief]

  [2]. Camillo Sargiacomo, et al. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Front Oncol. 2021 Jul 30;11:678343.  [Content Brief]