2. Antibody-drug Conjugate/ADC Related
  3. ADC Cytotoxin
  4. DC0-NH2

DC0-NH2 is an effector moiety for ADC and a simplified analog of DC1 with better stability. DC0-NH2 is about 1000-fold more cytotoxic than commonly used anticancer agents (ex. Doxorubicin). DC0-NH2 can bind to the minor groove of DNA, followed by alkylation of adenine residues by its propabenzindole (CBI) component.

For research use only. We do not sell to patients.

DC0-NH2 Chemical Structure

DC0-NH2 Chemical Structure

CAS No. : 615538-51-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1186 In-stock
Solution
10 mM * 1 mL in DMSO USD 1186 In-stock
Solid
1 mg USD 550 In-stock
5 mg USD 980 In-stock
10 mg USD 1500 In-stock
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Based on 1 publication(s) in Google Scholar

DC0-NH2 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

DC0-NH2 is an effector moiety for ADC and a simplified analog of DC1 with better stability. DC0-NH2 is about 1000-fold more cytotoxic than commonly used anticancer agents (ex. Doxorubicin). DC0-NH2 can bind to the minor groove of DNA, followed by alkylation of adenine residues by its propabenzindole (CBI) component[1].

IC50 & Target[1]

Duocarmycins

 

In Vitro

DC0-NH2 (0-3 nM; 72 hours) is highly potent against Ramos, Namalwa, and HL60/s cells with IC50 values in the 1 pM to 10 pM range, and has 100 pM range when tested on COLO 205 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DC0-NH2 Related Antibodies

Cell Viability Assay[1]

Cell Line: Ramos, Namalwa, HL60/s, and COLO 205 Cancer Cells
Concentration: 0-3 nM
Incubation Time: 72 hours
Result: Inhibited Namalwa and HL60/s cells with IC50s of 7 and 30 pM, respectively.
Molecular Weight

550.01

Formula

C31H24ClN5O3
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=C(C(N1)=CC2=C1C=CC(N)=C2)NC3=CC4=C(NC(C(N5C[C@@H](CCl)C6=C5C=C(O)C7=CC=CC=C67)=O)=C4)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (90.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8181 mL 9.0907 mL 18.1815 mL
5 mM 0.3636 mL 1.8181 mL 3.6363 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8181 mL 9.0907 mL 18.1815 mL 45.4537 mL
5 mM 0.3636 mL 1.8181 mL 3.6363 mL 9.0907 mL
10 mM 0.1818 mL 0.9091 mL 1.8181 mL 4.5454 mL
15 mM 0.1212 mL 0.6060 mL 1.2121 mL 3.0302 mL
20 mM 0.0909 mL 0.4545 mL 0.9091 mL 2.2727 mL
25 mM 0.0727 mL 0.3636 mL 0.7273 mL 1.8181 mL
30 mM 0.0606 mL 0.3030 mL 0.6060 mL 1.5151 mL
40 mM 0.0455 mL 0.2273 mL 0.4545 mL 1.1363 mL
50 mM 0.0364 mL 0.1818 mL 0.3636 mL 0.9091 mL
60 mM 0.0303 mL 0.1515 mL 0.3030 mL 0.7576 mL
80 mM 0.0227 mL 0.1136 mL 0.2273 mL 0.5682 mL
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DC0-NH2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DC0-NH2615538-51-7ADC CytotoxinADC PayloadCytotoxinADCCPIantibioticInhibitorinhibitorinhibit

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