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Art. -Nr.: HY-146321

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ANO1-IN-2

ANO1-IN-2 (Compound 10q) is a selective ANO1 channel blocker with an IC₅₀ of 1.75 μM and 7.43 μM against ANO1 and ANO2, respectively. ANO1-IN-2 suppresses strongly proliferation of glioblastoma cells.

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CAS. Nr.: 637314-12-6
Formel: C₁₆H₁₄ClN₃O₂S₂
Molecular Weight:379.88

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (268 KB)

ANO1-IN-2 Chemical Structure

CAS. Nr.: 637314-12-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Protokoll (2)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC₅₀: 1.75 μM (ANO1), 7.43 μM (ANO2)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Astrocyte	GI₅₀	45.15 μM Compound: 10q	Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay Antiproliferative activity against mouse primary astrocytes assessed as reduction in cell viability measured after 72 hrs by Celltiter-Glo luminescent assay	[PMID: 32906067]
U138-MG	GI₅₀	13.22 μM Compound: 10q	Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-251	GI₅₀	12.04 μM Compound: 10q	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]
U-87MG ATCC	GI₅₀	23.77 μM Compound: 10q	Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	[PMID: 32906067]

In Vitro

ANO1-IN-2 (Compound 10q) (10 μM) significantly suppresses migration and invasion of U251 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS. Nr. 637314-12-6
Molecular Weight 379.88
Formel C₁₆H₁₄ClN₃O₂S₂
SMILES

NC(C1=C(SC2=C1CCC2)NC(NC(C3=CC=C(C=C3)Cl)=O)=S)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Protokoll

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Choi SH, et al. Anti-glioma effects of 2-aminothiophene-3-carboxamide derivatives, ANO1 channel blockers. Eur J Med Chem. 2020 Dec 15;208:112688. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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