Art. -Nr.: HY-147140

BOS-318

Name: BOS-318
Brand: MedChemExpress
SKU: HY-147140
Price: 120.00 USD
Availability: InStock
Rating: 5 (3 reviews)

3 Cited Publications

Kundenbewertung

Based on 3 publication(s) in Google Scholar

BOS-318 is a potent, slowly dissociating, highly selective, and cell-permeable furin inhibitor with IC₅₀ value of 1.9 nM. BOS-318 protects ENaC from subsequent activation by neutrophil elastase. BOS-318 can be used for the rsearch for CF lung disease.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit : 99.24%
CAS. Nr.: 2387633-15-8
Formel: C₂₈H₃₂Cl₂N₆O₃
Molecular Weight:571.50

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

BOS-318 Chemical Structure

CAS. Nr.: 2387633-15-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (3)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

BOS-318 (0.001-1 μM, 72 hours) provides cytoprotection against toxicity in CuFi-1 cells induced by P aeruginosa exotoxin A (PEA)^[1].
BOS-318 (U2OS cells) to be approximately 13 times more potent against furin than PCSK5 (IC₅₀ = 25.3 nM), 24 times more potent than PCSK7 (IC₅₀ = 45.8 nM), and 110 times more potent than PCSK6 (IC₅₀ = 209.4 nM) ^[1].
BOS-318 (0.3 μM, 2 hours or 48 hours) reduces ENaC-mediated Na⁺ absorption in CF HBECs and protects against NE- and CF sputum-sol-mediated activation of the ENaC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

CuFi-1 cells
Concentration:

17.4 and 263.0 nM
Incubation Time:

2 and 48 hours
Result:

Significantly inhibited ENaC-mediated Na⁺ transport as measured in differentiated human bronchial epithelial cells under both short- (IC₅₀= 263.0 nM) and long-term (IC₅₀= 17.4 nM) treatment conditions.

Cell Viability Assay^[1]

Cell Line:

CuFi-1 cells
Concentration:

0.3 μM
Incubation Time:

72 hours
Result:

Significantly increased cell viability in PEA-treated cells to approximately 88% of control levels.

Cell Cytotoxicity Assay^[1]

Cell Line:

CuFi-1 cells
Concentration:

0.001, 0.01, 0.03, 0.1, 0.3 and 1 μM
Incubation Time:

72 hours
Result:

Provided protection against PEA-mediated cytotoxicity in a dose-dependent manner, over a period of 72 h, with an EC50 of 47.8 nM.

Related Protocols :

Phagocytosis Functional Assay

Chemical Information

CAS. Nr. 2387633-15-8
Appearance Solid
Molecular Weight 571.50
Formel C₂₈H₃₂Cl₂N₆O₃
Color Off-white to light yellow
SMILES

O=C(O)CC1CCN(CC2=CC(C3=CC(Cl)=CC(Cl)=C3)=NC(OC4=CN=C(N5CCN(C)CC5)N=C4)=C2)CC1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

All WBFlow Cytometry

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Journal Impact Factor

Acta Biomater

Fe3+-responsive aggregable gold nanoplatform enables siRNA-mediated chemoresistance reversal and combined photothermal-temozolomide therapy for glioblastoma. [Abstract]

2026 Jun 18:S1742-7061(26)00397-1. PMID: 42315000
Sci Rep

The unexplored mechanism of antitumoral effect of pirfenidone in melanoma cells. [Abstract]

2025 Aug 1;15(1):28071. PMID: 40751072

BOS-318 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Aug 1;15(1):28071. [Abstract]

Western blotting analysis of p21 in M21 melanoma cells exposed to 2.7 mM and 8.1 mM concentration of PFD and/or furin inhibitor BOS318 (5 nM).

BOS-318 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Aug 1;15(1):28071. [Abstract]

The cotreatment with PFD 8.1 mM and BOS318 (5 nM, 24 h) synergistically cooperate to the increased percentage of melanoma cells in late apoptosis.

BOS-318 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Aug 1;15(1):28071. [Abstract]

Western blotting of intracellular TGFβ (44 kDa) in A375M6, and M21 human melanoma exposed to PFD at 2.7 mM and 8.1 mM concentrations, in the presence or in the absence of BOS318 (5 nM, 24 h). Each panel of the figure shows a typical experiment out of three that gave similar results.
Npj Viruses

Site-1 protease mediated GPC processing is required for persistence of LCMV Clone 13. [Abstract]

2026 Mar 14;4(1):18. PMID: 41826412

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (87.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7498 mL

8.7489 mL

17.4978 mL

5 mM

0.3500 mL

1.7498 mL

3.4996 mL

10 mM

0.1750 mL

0.8749 mL

1.7498 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Douglas LEJ, et al. A highly selective, cell-permeable furin inhibitor BOS-318 rescues key features of cystic fibrosis airway disease. Cell Chem Biol. 2022 Jun 16;29(6):947-957.e8. [Content Brief]

[2]. Nayak D, et al. Clearing the air: Uniquely engaging furin as an approach to cystic fibrosis therapy. Cell Chem Biol. 2022 Jun 16;29(6):927-929. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7498 mL	8.7489 mL	17.4978 mL	43.7445 mL
	5 mM	0.3500 mL	1.7498 mL	3.4996 mL	8.7489 mL
	10 mM	0.1750 mL	0.8749 mL	1.7498 mL	4.3745 mL
	15 mM	0.1167 mL	0.5833 mL	1.1665 mL	2.9163 mL
	20 mM	0.0875 mL	0.4374 mL	0.8749 mL	2.1872 mL
	25 mM	0.0700 mL	0.3500 mL	0.6999 mL	1.7498 mL
	30 mM	0.0583 mL	0.2916 mL	0.5833 mL	1.4582 mL
	40 mM	0.0437 mL	0.2187 mL	0.4374 mL	1.0936 mL
	50 mM	0.0350 mL	0.1750 mL	0.3500 mL	0.8749 mL
	60 mM	0.0292 mL	0.1458 mL	0.2916 mL	0.7291 mL
	80 mM	0.0219 mL	0.1094 mL	0.2187 mL	0.5468 mL

Bioz

Keywords

BOS-318

3 Cited Publications

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BOS-318

Publications Citing Use of MedChemExpress (MCE) BOS-318