Art. -Nr.: HY-N9968

Cucurbitacin C

Name: Cucurbitacin C
Brand: MedChemExpress
SKU: HY-N9968
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(Synonyms: CuC)

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Cucurbitacin C is a tetracyclic triterpenoid compound. Cucurbitacin C exhibits significant in vivo and in vitro anticancer activity, which inhibits the PI3K/AKT signaling pathway to induce cell cycle arrest and apoptosis in cancer cells, and significantly suppresses the growth of HepG2 and PC-3 xenograft tumors in mice. Cucurbitacin C can be used in studies on plant defense mechanisms, secondary metabolism and cancer therapy.

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Reinheit: 95.0%
CAS. Nr.: 5988-76-1
Formel: C₃₂H₄₈O₈
Molecular Weight:560.72

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Cucurbitacin C Chemical Structure

CAS. Nr.: 5988-76-1

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Akt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC50	0.21 μM	Antiproliferative activity against human HCT-116 colon cancer cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay. Antiproliferative activity against human HCT-116 colon cancer cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay.	38505422
HCT-116	IC50	0.04 μM	Antiproliferative activity against human HCT-116 colon cancer cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay. Antiproliferative activity against human HCT-116 colon cancer cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay.	38505422
PC-3	IC50	19.6 nM	Antiproliferative activity against human prostate cancer PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay. Antiproliferative activity against human prostate cancer PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.	30707394
LNCaP	IC50	158.7 nM	Antiproliferative activity against human prostate cancer LNCaP cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay. Antiproliferative activity against human prostate cancer LNCaP cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.	30707394

In Vitro

Cucurbitacin C (CuC) (0.01-10 μM; 24-48 h) potently inhibits the proliferation of HCT-116 colon cancer cells, with an IC₅₀ value of 0.21 μM at 24 h and 0.04 μM at 48 h^[4].
Cucurbitacin C (0.1-0.5 μM; 24 h) inhibits the migration of HCT-116 colon cancer cells in a concentration-dependent manner^[4].
Cucurbitacin C (24 h) downregulates the mRNA expression of MMP-1, MMP-3, MMP-9, MMP-13 and PGF in HCT-116 cells, exerts no significant effect on CDK2 expression, and upregulates MET expression^[4].
Cucurbitacin C (10-100 nM; 14 days) significantly reduces the clonogenic capacity of LNCaP, DU145, PC-3, T24 and HepG2 cells^[1].
Cucurbitacin C (20 nM; 12-24 h) significantly inhibits the migration of DU145, PC-3, HepG2 and T24 cells^[1].
Cucurbitacin C (100 nM; 24 h) inhibits phosphorylation of Akt at the Ser473 site in a time-dependent manner in PC-3, T24 and HepG2 cells, with no effect on the expression of pan-Akt^[1].
Cucurbitacin C exhibits favorable binding affinity to human MMP-1, MMP-3, MMP-9, MMP-13, PGF, MET and CDK2 proteins^[4].
Cucurbitacin C (49.5-314.4 μg/g fresh weight; at approximately 10 days) shows an extremely strong negative correlation with the survival rate of Tetranychus urticae in bitter haploid cucumber lines^[5].
Cucurbitacin C (0.1-1.0 μM; 24 h) induces concentration-dependent apoptosis in HCT-116 colon cancer cells after 24 h of treatment^[4].
Cucurbitacin C (10-1000 nM; 24 h) induces dose-dependent early apoptosis in T24, HepG2 and PC-3 cells^[1].
Cucurbitacin C (0.01-1 μM; 48 h) activates apoptosis-related proteins in LNCaP, PC-3, T24 and HepG2 cells through distinct pathways, including activation of caspase-8 and PARP in PC-3 and T24 cells, promotion of cleaved caspase-3 accumulation in LNCaP cells, and upregulation of cleaved caspase-9 levels in HepG2 cells^[1].
Cucurbitacin C (10-1000 nM; 48 h) induces G1 phase arrest in DU145 and LNCaP cells, triggers G2/M phase arrest in T24, HepG2 and PC-3 cells, and increases the proportion of apoptotic cells in the sub-G1 phase^[1].
Cucurbitacin C (100 nM; 24 h) regulates cell cycle regulatory proteins in LNCaP, PC-3, T24 and HepG2 cells, reduces the levels of cyclin A and cyclin D1, and induces p53-independent upregulation of p21^[1].
Cucurbitacin C (24 h) alters the gene expression of PC-3 and HepG2 cells, exerts significant regulatory effects on the PI3K-Akt pathway, and upregulates cell cycle inhibitors and downstream target genes of Akt^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

human HCT-116 colon cancer cells
Concentration:

0.01, 0.05, 0.1, 0.5, 1, 5, 10 μM
Incubation Time:

24, 48 h
Result:

Inhibited the proliferative activity of HCT-116 cells in a concentration- and time-dependent manner, with IC₅₀ values of 0.21 μM at 24 h and 0.04 μM at 48 h.

Apoptosis Analysis^[4]

Cell Line:

human HCT-116 colon cancer cells
Concentration:

0.1, 0.5, 1.0 μM
Incubation Time:

24 h
Result:

Induced concentration-dependent apoptosis in HCT-116 cells, with increasing proportions of early and late apoptotic cells observed at higher cucurbitacin C concentrations.

Cell Migration Assay ^[4]

Cell Line:

human HCT-116 colon cancer cells
Concentration:

0.1, 0.5, 1.0 μM
Incubation Time:

24 h
Result:

Inhibited migratory activity of HCT-116 cells in a concentration-dependent manner, with significantly reduced migration rate in the high-dose treatment group compared to controls.

Cell Viability Assay^[1]

Cell Line:

LNCaP, DU145, PC-3, T24, HepG2
Concentration:

0.001, 0.01, 0.1, 1, 10 μM
Incubation Time:

48 h
Result:

Dose-dependently inhibited the survival of all tested cancer cell lines.
Achieved 40-60% inhibition of cell survival at 10-100 nM, with PC-3 and T24 cells showing the highest sensitivity.

Cell Cycle Analysis^[1]

Cell Line:

DU145, LNCaP, PC-3, T24, HepG2
Concentration:

10, 100, 1000 nM
Incubation Time:

48 h
Result:

Induced G1 phase cell cycle arrest in DU145 and LNCaP cells.
Induced G2/M phase arrest in T24, HepG2, and PC-3 cells.
Caused a dramatic increase in the sub-G1 cell population at 1 μM.

Western Blot Analysis^[1]

Cell Line:

LNCaP, PC-3, T24, HepG2
Concentration:

0.01, 0.1, 1 μM
Incubation Time:

24 h
Result:

Dose-dependently up-regulated p21 protein levels in PC-3, T24, and HepG2 cells.
Caused p21 accumulation to peak at 1 μM in PC-3 cells and at 0.1 μM in HepG2 cells.
Showed no change in p53 expression in any of the cell lines.

Western Blot Analysis^[1]

Cell Line:

LNCaP, PC-3, T24, HepG2
Concentration:

0.01, 0.1, 1 μM
Incubation Time:

48 h
Result:

Dose-dependently activated caspase-8 and PARP in PC-3 and T24 cells.
Activated caspase-9 in PC-3 cells.
Slightly elevated cleaved caspase-3/-7 levels in PC-3 cells but not in T24 cells.

Western Blot Analysis^[1]

Cell Line:

PC-3, T24, HepG2
Concentration:

100 nM
Incubation Time:

24 h
Result:

Significantly decreased Akt phosphorylation at Ser473 in PC-3, T24, and HepG2 cells after 24 h, with no change in pan-Akt expression.

Western Blot Analysis^[1]

Cell Line:

PC-3, T24
Concentration:

100 nM
Incubation Time:

1, 3, 10, 24 h
Result:

Inhibited p-Akt (Ser473) in PC-3 cells starting at 3 h, with the most prominent effect at 24 h.
Inhibited p-Akt (Ser473) in T24 cells starting at 1 h, reaching the lowest point at 24 h.

In Vivo

Cucurbitacin C (0.05-0.1 mg/kg/day; i.p.; once daily for 21 days) inhibits colon tumor growth in mice with 41.94% and 50.45% tumor inhibition rates at 0.05 mg/kg/day and 0.1 mg/kg/day, respectively, while restoring weight loss and downregulating PGF and MMP family gene expression in tumor tissues^[4].
Cucurbitacin C (0.1 mg/kg; i.p.; three times per week; 4 weeks) inhibits HepG2 xenograft tumor growth by reducing tumor weight and inducing intratumor apoptosis with low host toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (male, 6-8 weeks old, 20 g)^[4]
Dosage:

0.05, 0.1 mg/kg
Administration:

i.p.; once daily for 21 days
Result:

Restored tumor-induced weight loss in mice.
Achieved tumor inhibition.
Reduced tumor weight and volume significantly compared to the model group for both doses.
Decreased the expression of MMP-1, MMP-3, MMP-9, MMP-13, and Pgf genes in tumor tissues for both doses.
Showed no significant effect on MET or CDK2 expression in tumor tissues for both doses.

Animal Model:

SCID (7-week-old male, HepG2 cell subcutaneous xenograft model)^[1]
Dosage:

0.1 mg/kg
Administration:

i.p.; three times per week; 4 weeks
Result:

Reduced average tumor weight.
Increased DNA cleavage signals in treated tumors compared to controls.
Caused no significant changes in body weight between treated and control mice.

Animal Model:

SCID (7-week-old male, PC-3 cell subcutaneous xenograft model)^[1]
Dosage:

0.1 mg/kg
Administration:

i.p.; three times per week; 4 weeks
Result:

Reduced average tumor weight.
Increased DNA cleavage signals in treated tumors compared to controls.
Caused no significant changes in body weight between treated and control mice.

Chemical Information

CAS. Nr. 5988-76-1
Appearance Solid
Molecular Weight 560.72
Formel C₃₂H₄₈O₈
Color White to off-white
SMILES

OC[C@]12[C@](CC=C3[C@@]2([H])CC[C@H](C3(C)C)O)([H])[C@]4([C@](CC1=O)([C@@]([C@@H](C4)O)([H])[C@@](C)(O)C(/C=C/C(C)(C)OC(C)=O)=O)C)C
Synonyms

CuC
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (306 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu D, et al. Corrigendum: In vitro and in vivo antitumor activity of cucurbitacin C, a novel natural product from cucumber. Front Pharmacol. 2024 Mar 4;15:1334971.

[2]. Liu Z, et al. The role of HS in low temperature-induced cucurbitacin C increases in cucumber. Plant molecular biology. 2019 Apr;99(6):535-544.

[3]. Hideki HORIE, et al. Cucurbitacin C—Bitter Principle in Cucumber Plants, Japan Agricultural Research Quarterly: JARQ, 2007, Volume 41, Issue 1, Pages 65-68, Released on J-STAGE March 26, 2013, Online ISSN 2185-8896, Print ISSN 0021-3551,

[4]. Liu, J, et al. Preliminary investigation of the anti-colon cancer activity of cucurbitacin C from cucumber: A network pharmacological study and experimental validation. Food Frontiers. 2024; 5, 142-159.

[5]. Balkema-Boomstra AG, et al. Role of cucurbitacin C in resistance to spider mite (Tetranychus urticae) in cucumber (Cucumis sativus L.). Journal of chemical ecology. 2003 Jan;29(1):225-35.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Cucurbitacin C

Alle Akt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Cucurbitacin C Related Classifications

HY-N9968 Related Classifications

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