country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Art. -Nr.: HY-P11781

Copy Link Print Information

HW12

HW12 is a scrambled peptide control with no EGFR ligand activity. HW12 shows low cellular uptake levels in EGFR-positive cells. HW12 exerts weak growth inhibitory effects on pancreatic cancer. HW12 serves as a scrambled peptide control for EGFR-targeted GE11 (HY-P10128)-conjugated mixed micelles. HW12 can be used in pancreatic cancer research.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 875142-27-1
Formel: C₇₂H₈₉N₁₉O₁₇
Molecular Weight:1492.59

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Kundenspezifische Peptidsynthese

HW12 Chemical Structure

CAS. Nr.: 875142-27-1

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle EGFR Isoform-spezifische Produkte anzeigen :

In Vitro

The HW12 peptide can be successfully conjugated with MAL-PEG-PCD via a thiol-maleimide addition reaction to form HW12-PEG-PCD, and this result is confirmed by ¹H NMR analysis^[1].
The cellular uptake of FC-labeled HW12-linked mixed micelles (12 h) by MIA PaCa-2 cells is significantly lower than that of GE11-conjugated mixed micelles, indicating that HW12 cannot mediate EGFR-specific cellular uptake^[1].
HW12-linked mixed micelles (60-500 nM GEM; 72 h) exhibit lower cytotoxicity against MIA PaCa-2 cells than GE11-linked mixed micelles, with an IC₅₀ of 126.58 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

MIA PaCa-2 pancreatic cancer cells
Concentration:

HW12-linked mixed micelles with GEM concentrations of 60, 500 nM
Incubation Time:

72 h
Result:

Showed lower cytotoxicity than GE11-linked mixed micelles and unmodified micelles at all tested GEM concentrations.
Reached an IC₅₀ value of 126.58 nM, which was 1.3-fold higher than the IC₅₀ of GE11-linked mixed micelles (95.32 nM) and 1.1-fold higher than the IC₅₀ of unmodified micelles (116.91 nM).

In Vivo

HW12-linked mixed micelles (40 mg/kg equivalent dose of gemcitabine; i.v.; once weekly; 3 weeks) deliver gemcitabine but show minimal orthotopic pancreatic tumor growth inhibition in athymic nude mice, with significantly lower efficacy than EGFR-targeted GE11-linked mixed micelles^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

athymic nude mice (6-8 week-old)^[1]
Dosage:

40 mg/kg equivalent dose of gemcitabine
Administration:

i.v.; once weekly; 3 weeks
Result:

Showed minimal tumor growth inhibition compared to GE11-linked mixed micelles.
Resulted in significantly higher tumor bioluminescence, volume, and weight than in GE11-linked mixed micelle-treated mice.
Induced more Ki-67 staining, less cleaved caspase-3 staining, fewer necrotic tumor areas, less TUNEL-positive tumor and endothelial cell apoptosis, and higher tumor microvessel density compared to GE11-linked mixed micelles.
Caused no abnormal body weight changes.
Led to no pathological changes detected in major organs (heart, liver, kidney).

Chemical Information

CAS. Nr. 875142-27-1
Molecular Weight 1492.59
Formel C₇₂H₈₉N₁₉O₁₇
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Mondal G, et al. EGFR-Targeted Polymeric Mixed Micelles Carrying Gemcitabine for Treating Pancreatic Cancer. Biomacromolecules. 2016;17(1):301-313. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Angebot einholen Auf Lager

Other size

Angebot einholen

Amount: USD 0.00

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

HW12

Alle EGFR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

HW12 Related Classifications

HY-P11781 Related Classifications