DPBQ
Based on 1 publication(s) in Google Scholar
DPBQ activates p53 and triggers apoptosis in a polyploid-specific manner, but does not inhibit topoisomerase or bind DNA. DPBQ elicits expression and phosphorylation of p53 and this effect is specific to tetraploid cells.
For research use only. We do not sell to patients.
- Purity: 99.46%
- CAS No.: 7029-89-2
- Formula: C24H14N2O2
- Molecular Weight:362.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) DPBQ
More
Biological Activity
p53[1]
DPBQ elicits apoptosis and cell death and is selective for effects in 4N cells. DPBQ elicits expression and phosphorylation of p53 and this effect is specific to tetraploid cells. Knockdown of TP53 restores proliferation of tetraploid cells in the presence of DPBQ[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7029-89-2
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Appearance Solid
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Molecular Weight 362.38
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Formula C24H14N2O2
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Color Light yellow to green yellow
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SMILES
O=C(C1=CC=CC=C12)C3=C(N=C(C4=CC=CC=C4)C(C5=CC=CC=C5)=N3)C2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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EBioMedicine
Exercise downregulates HIPK2 and HIPK2 inhibition protects against myocardial infarction. [Abstract]2021 Dec:74:103713. PMID: 34837851
Solvent & Solubility
DMSO : 2.94 mg/mL (8.11 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
For Western blot analysis, 2N and 4N RPE1 cells are treated with DMSO, 50 ng/mL doxorubicin, or 1 μM DPBQ for 48 hours. Western blotting is performed with a SDS-PAGE Electrophoresis and semidry transfer. Samples are analyzed by chemiluminescence. β-actin is used as a protein loading control[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
For Apoptosis assays, 2N and 4N RPE1 cells are treated with DMSO, 60 nM staurosporine, or 1 μM DPBQ for 48 hours. Apoptosis is evaluated by an Annexin V-PE/7-AAD apoptosis detection kit. For the flow cytometry analysis, the percent positive cells in the upper right and lower right quadrants are summed to give the total number of apoptotic cells. Three independent replicates are performed[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7595 mL | 13.7977 mL | 27.5953 mL | 68.9884 mL |
| 5 mM | 0.5519 mL | 2.7595 mL | 5.5191 mL | 13.7977 mL |
DPBQ Related Classifications
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