CYT296
Based on 1 Customer Validation
CYT296 is a target chromatin de-condensation compound. CYT296 can improve the induction of induced pluripotent stem cell (iPSCs) mediated by defined factors (OSKM) and induce an open chromatin state in Mouse Embryonic Fibroblast (MEFs) to facilitate somatic cell reprogramming. CYT296 can be used for cell replacement therapies and drug screening research.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 1799392-31-6
- Formula: C18H16O2S
- Molecular Weight:296.38
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
CYT296 (250nM, 72 h) changes the MEF chromatin and allows easier access of Yamanaka factors and facilitates the reprogramming process[1].
CYT296 (250nM, 72 h) block heterochromatin assembly of MEFs to improve the generation of iPSC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MEFs
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Concentration:250 nM
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Incubation Time:72 h
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Result:Reduced the number and total intensity of HP1α+ and H3K9me3+ foci in the nuclei of MEFs.
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Cell Line:MEFs
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Concentration:250 nM
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Incubation Time:72 h
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Result:Speeded up the expression of Nanog during 4F-mediated reprogramming and appeared as early as day 8.
Reduced the protein level of HP1α during reprogramming.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4F- and 2F-iPSC clones mice[1]
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Dosage:10-15 4F- and 2F -iPSC clones
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Administration:Subcutaneous injection (s.c.)
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Result:Obtained 6 out of 10 born mice from clone 4F-1 and 9 out of 15 born mice from clone 2F-1 were chimeras.
Chemical Information
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CAS No. 1799392-31-6
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Appearance Oil
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Molecular Weight 296.38
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Formula C18H16O2S
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Color White to off-white
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SMILES
O=C1[C@@]2(CSC3=CC=CC=C31)[C@H](C4=CC=C(C=C4)OC)C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (337.40 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.44 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.44 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3740 mL | 16.8702 mL | 33.7405 mL | 84.3512 mL |
| 5 mM | 0.6748 mL | 3.3740 mL | 6.7481 mL | 16.8702 mL | |
| 10 mM | 0.3374 mL | 1.6870 mL | 3.3740 mL | 8.4351 mL | |
| 15 mM | 0.2249 mL | 1.1247 mL | 2.2494 mL | 5.6234 mL | |
| 20 mM | 0.1687 mL | 0.8435 mL | 1.6870 mL | 4.2176 mL | |
| 25 mM | 0.1350 mL | 0.6748 mL | 1.3496 mL | 3.3740 mL | |
| 30 mM | 0.1125 mL | 0.5623 mL | 1.1247 mL | 2.8117 mL | |
| 40 mM | 0.0844 mL | 0.4218 mL | 0.8435 mL | 2.1088 mL | |
| 50 mM | 0.0675 mL | 0.3374 mL | 0.6748 mL | 1.6870 mL | |
| 60 mM | 0.0562 mL | 0.2812 mL | 0.5623 mL | 1.4059 mL | |
| 80 mM | 0.0422 mL | 0.2109 mL | 0.4218 mL | 1.0544 mL | |
| 100 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8435 mL |
CYT296 Related Classifications
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