DiFMUP
Based on 2 publication(s) in Google Scholar
DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader.
For research use only. We do not sell to patients.
- Purity: 98.86%
- CAS No.: 214491-43-7
- Formula: C10H7F2O6P
- Molecular Weight:292.13
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DiFMUP
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Biological Activity
DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 214491-43-7
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Appearance Solid
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Molecular Weight 292.13
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Formula C10H7F2O6P
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Color White to off-white
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SMILES
O=P(OC1=C(C(OC2=O)=C(C(C)=C2)C=C1F)F)(O)O
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Synonyms
6,8-Difluoro-4-methylumbelliferyl phosphate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Discovery of a Potent Fluorescence Polarization Probe for Identifying USP1 Allosteric Inhibitors. [Abstract]2026 Jul;13(39):e75350. PMID: 42017803 -
Eur J Med Chem
Discovery of novel substituted pyridine carboxamide derivatives as potent allosteric SHP2 inhibitors. [Abstract]2024 Sep 5:279:116830. PMID: 39303516
Solvent & Solubility
DMSO : 50 mg/mL (171.16 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8. [Content Brief]
[2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4231 mL | 17.1157 mL | 34.2313 mL | 85.5783 mL |
| 5 mM | 0.6846 mL | 3.4231 mL | 6.8463 mL | 17.1157 mL | |
| 10 mM | 0.3423 mL | 1.7116 mL | 3.4231 mL | 8.5578 mL | |
| 15 mM | 0.2282 mL | 1.1410 mL | 2.2821 mL | 5.7052 mL | |
| 20 mM | 0.1712 mL | 0.8558 mL | 1.7116 mL | 4.2789 mL | |
| 25 mM | 0.1369 mL | 0.6846 mL | 1.3693 mL | 3.4231 mL | |
| 30 mM | 0.1141 mL | 0.5705 mL | 1.1410 mL | 2.8526 mL | |
| 40 mM | 0.0856 mL | 0.4279 mL | 0.8558 mL | 2.1395 mL | |
| 50 mM | 0.0685 mL | 0.3423 mL | 0.6846 mL | 1.7116 mL | |
| 60 mM | 0.0571 mL | 0.2853 mL | 0.5705 mL | 1.4263 mL | |
| 80 mM | 0.0428 mL | 0.2139 mL | 0.4279 mL | 1.0697 mL | |
| 100 mM | 0.0342 mL | 0.1712 mL | 0.3423 mL | 0.8558 mL |
DiFMUP Related Classifications
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