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  4. Diethyl succinate

Diethyl succinate  (Synonyms: Diethyl Butanedioate)

Cat. No.: HY-Y0836 Purity: 98.0%
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Diethyl succinate (Diethyl Butanedioate) can be utilized at physiological pH, allowing it to penetrate biological membranes and integrate into the cells of tissue cultures, where it is metabolized via the tricarboxylic acid cycle. Diethyl succinate modulates the polarization and activation of microglial cells by reducing mitochondrial fission and the levels of reactive oxygen species (ROS), thereby exerting an inflammatory protective effect in primary microglial cells. Furthermore, Diethyl succinate is non-toxic and can be used in flavorings and seasonings.

For research use only. We do not sell to patients.

CAS No. : 123-25-1

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Liquid
100 mg In-stock
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1 g In-stock
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10 g In-stock
50 g   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Diethyl succinate:

Diethyl succinate Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
Bio/Physico-chemical Assay

    Diethyl succinate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 28:S1097-2765(25)00819-6.  [Abstract]

    HeLa cells treated with either vehicle (ethanol) or Diethyl succinate (DES, 3 mM) were monitored for proliferation over 4 days. Prolonged exposure to DES significantly impaired proliferation, highlighting that chronic succinate accumulation negatively impacts cell growth.

    Diethyl succinate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 28:S1097-2765(25)00819-6.  [Abstract]

    Fractional enrichment (%) of the indicated isotopologues from HeLa cells labeled with [15N-13C2]-glycine for 4 h in the presence or absence of exogenous cell-permeable succinate (Diethyl succinate [DES]). Stable isotope tracing using [[15N-13C2]-glycine revealed a marked decrease in labeled purine nucleotides, including AMP (M+3) and GMP (M+3), following DES treatment.

    Diethyl succinate purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Oct 28:S1097-2765(25)00819-6.  [Abstract]

    The relative levels of incorporation of [14C] into RNA are shown. HeLa cells were treated with either vehicle or Diethyl succinate (DES, 5 mM) for 8 h. DES treatment decreased the incorporation of [14C] from glycine into RNA.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Diethyl succinate (Diethyl Butanedioate) can be utilized at physiological pH, allowing it to penetrate biological membranes and integrate into the cells of tissue cultures, where it is metabolized via the tricarboxylic acid cycle. Diethyl succinate modulates the polarization and activation of microglial cells by reducing mitochondrial fission and the levels of reactive oxygen species (ROS), thereby exerting an inflammatory protective effect in primary microglial cells. Furthermore, Diethyl succinate is non-toxic and can be used in flavorings and seasonings[1][2].

    In Vitro

    Diethyl succinate (5 mM; 3 h) reduces ROS production in primary microglia[2].
    Diethyl succinate (0-10 mM; 0.25-3 h) induces decreased mitochondrial fission in primary microglia[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Diethyl succinate Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Primary microglia
    Concentration: 0, 0.2, 0.5, 1, 5 and 10 mM
    Incubation Time: 0.25, 1 and 3 h
    Result: Increased phosphorylation of DRP1 at S637.
    Molecular Weight

    174.19

    Formula

    C8H14O4
    CAS No.
    Appearance

    Liquid (Density: 1.047 g/cm3)

    Color

    Colorless to light yellow

    SMILES

    O=C(OCC)CCC(OCC)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 250 mg/mL (1435.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7409 mL 28.7043 mL 57.4086 mL
    5 mM 1.1482 mL 5.7409 mL 11.4817 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (11.94 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.98%

    SDS (393 KB)

    COA (242 KB) HNMR (127 KB) GC (178 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 5.7409 mL 28.7043 mL 57.4086 mL 143.5214 mL
    5 mM 1.1482 mL 5.7409 mL 11.4817 mL 28.7043 mL
    10 mM 0.5741 mL 2.8704 mL 5.7409 mL 14.3521 mL
    15 mM 0.3827 mL 1.9136 mL 3.8272 mL 9.5681 mL
    20 mM 0.2870 mL 1.4352 mL 2.8704 mL 7.1761 mL
    25 mM 0.2296 mL 1.1482 mL 2.2963 mL 5.7409 mL
    30 mM 0.1914 mL 0.9568 mL 1.9136 mL 4.7840 mL
    40 mM 0.1435 mL 0.7176 mL 1.4352 mL 3.5880 mL
    50 mM 0.1148 mL 0.5741 mL 1.1482 mL 2.8704 mL
    60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3920 mL
    80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7940 mL
    100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4352 mL
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    Diethyl succinate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Diethyl123-25-1Diethyl ButanedioateBiochemical Assay ReagentsReactive Oxygen Species (ROS)Environmental PollutantsEnvironmental ContaminantsInhibitorinhibitorinhibit

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