Cat. No.: HY-N2532

Diphyllin

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Diphyllin is an orally active V-ATPase inhibitor (IC₅₀=17 nM) and HIV-1 inhibitor (IC₅₀=0.38 μM). Diphyllin blocks the acidification of osteoclast lysosomes and bone resorption lacunas (IC₅₀=0.6 nM for acid influx inhibition)， thereby inhibiting bone resorption. Diphyllin can effectively inhibit osteoclast-mediated bone resorption and has no effect on osteoblastic bone formation. Diphyllin can be used in the research of bone metabolism-related diseases and has the potential to inhibit diseases related to excessive bone resorption.

For research use only. We do not sell to patients.

Purity: 99.57%
CAS No.: 22055-22-7
Formula: C₂₁H₁₆O₇
Molecular Weight:380.35

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Diphyllin Chemical Structure

CAS No.: 22055-22-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]

HIV-1

0.38 μM (IC₅₀)

Vacuolar type H⁺-ATPase

17 nM (IC₅₀)

Vesicular stomatitis virus (VSV)

All HIV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2058	IC₅₀	2.6 μM Compound: 1	Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
A549	IC₅₀	0.89 μM Compound: Diphyllin	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 38336655]
A549	IC₅₀	2.02 μM Compound: 1	Cytotoxicity against human A549 cells incubated for 24 hrs by MTS assay Cytotoxicity against human A549 cells incubated for 24 hrs by MTS assay	[PMID: 36857518]
A549	IC₅₀	24.01 μM Compound: 1	Cytotoxicity against human A549 cells measured after 3 days by MTT assay Cytotoxicity against human A549 cells measured after 3 days by MTT assay	[PMID: 36857518]
A549	IC₅₀	8 μM Compound: 5a	Anticancer activity against Homo sapiens (human) A549 cells assessed as decrease in cell growth after 3 days by MTT assay Anticancer activity against Homo sapiens (human) A549 cells assessed as decrease in cell growth after 3 days by MTT assay	10.1007/s00044-012-0245-1
A549	IC₅₀	8 μM Compound: Diphyllin	Cytotoxicity against Homo sapiens (human) A549 cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) A549 cells after 72 hr by MTT assay	10.1007/s00044-011-9937-1
A549	IC₅₀	8.8 μM Compound: 1	Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
Calu-1	IC₅₀	31.8 μM Compound: 1	Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
CFPAC-1	IC₅₀	0.208 μM Compound: 1	Antiproliferative activity against human CFPAC-1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay Antiproliferative activity against human CFPAC-1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay	[PMID: 36122549]
DU-145	IC₅₀	0.38 μM Compound: Diphyllin	Antiproliferative activity against human DU-145 cells assessed as inhibition of cell growth after 72 hrs by MTT assay Antiproliferative activity against human DU-145 cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 38336655]
EBC-1	IC₅₀	14.8 μM Compound: 1	Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
ECa-109 cell line	IC₅₀	0.21 μM Compound: 1	Antiproliferative activity against human Eca-109 cells measured after 2 days by CCK-8 method Antiproliferative activity against human Eca-109 cells measured after 2 days by CCK-8 method	[PMID: 36857518]
HCT-116	ED₅₀	0.8 μg/mL Compound: 3	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 11908984]
HCT-116	IC₅₀	3.54 μM Compound: 1	Cytotoxicity against human HCT116 cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
HCT-116	IC₅₀	48.5 μM Compound: 5a	Anticancer activity against Homo sapiens (human) HCT116 cells assessed as decrease in cell growth after 3 days by MTT assay Anticancer activity against Homo sapiens (human) HCT116 cells assessed as decrease in cell growth after 3 days by MTT assay	10.1007/s00044-012-0245-1
HEK293	CC₅₀	>80 μM Compound: Diphyllin	Cytotoxicity against HEK293 cells incubated for 72 hrs by MTT assay Cytotoxicity against HEK293 cells incubated for 72 hrs by MTT assay	[PMID: 38875806]
HEK293	IC₅₀	>100 μM Compound: 5a	Cytotoxicity against Homo sapiens (human) HEK293 cells assessed as decrease in cell growth after 3 days by MTT assay Cytotoxicity against Homo sapiens (human) HEK293 cells assessed as decrease in cell growth after 3 days by MTT assay	10.1007/s00044-012-0245-1
HEK293	IC₅₀	774.4 μM Compound: Diphyllin	Cytotoxicity against Homo sapiens (human) HEK293 cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) HEK293 cells after 72 hr by MTT assay	10.1007/s00044-011-9937-1
Hep 3B2	ED₅₀	3.6 μg/mL Compound: 3	Cytotoxicity against human Hep3B cells by MTT assay Cytotoxicity against human Hep3B cells by MTT assay	[PMID: 11908984]
HepG2	ED₅₀	0.4 μg/mL Compound: 3	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 11908984]
HepG2	IC₅₀	>500 μM Compound: 1	Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay	10.1007/s00044-009-9172-1
HepG2	IC₅₀	21.6 μM Compound: 1	Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
HT-29	ED₅₀	2.5 μg/mL Compound: 3	Cytotoxicity against human HT-29 cells by MTT assay Cytotoxicity against human HT-29 cells by MTT assay	[PMID: 11908984]
HT-29	IC₅₀	19.5 μM Compound: 1	Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
HT-29	IC₅₀	7.6 μM Compound: 8	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 24937209]
K562	IC₅₀	1.67 μM Compound: 1	Cytotoxicity against human K562 cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against human K562 cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
K562	IC₅₀	2.38 μM Compound: Diphyllin	Cytotoxicity against human etoposide-sensitive K562 cells after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay Cytotoxicity against human etoposide-sensitive K562 cells after 72 hrs by CellTiter96 AQueous one solution cell proliferation assay	[PMID: 29656990]
K562	IC₅₀	23.46 μM Compound: 1	Cytotoxicity against adriamycin-selected multi drug-resistant human K562 cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against adriamycin-selected multi drug-resistant human K562 cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
KB	ED₅₀	<1 μg/mL Compound: 10	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 2849640]
KB	ED₅₀	<1 μg/mL Compound: 2	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 3734816]
KB	IC₅₀	0.78 μM Compound: 1	Cytotoxicity against human KB cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against human KB cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
KB	IC₅₀	3.15 μM Compound: 1	Cytotoxicity against vincristine-selected multi drug-resistant human KB cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against vincristine-selected multi drug-resistant human KB cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
KB	IC₅₀	5.3 μM Compound: 5a	Anticancer activity against Homo sapiens (human) KB cells assessed as decrease in cell growth after 3 days by MTT assay Anticancer activity against Homo sapiens (human) KB cells assessed as decrease in cell growth after 3 days by MTT assay	10.1007/s00044-012-0245-1
KB	IC₅₀	5.3 μM Compound: Diphyllin	Cytotoxicity against Homo sapiens (human) KB cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) KB cells after 72 hr by MTT assay	10.1007/s00044-011-9937-1
L02	IC₅₀	3.322 μM Compound: 1	Antiproliferative activity against human L02 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay Antiproliferative activity against human L02 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay	[PMID: 36122549]
LNCaP	IC₅₀	11.5 μM Compound: 6	Cytotoxicity against androgen sensitive human LNCaP cells Cytotoxicity against androgen sensitive human LNCaP cells	[PMID: 17256902]
MCF7	IC₅₀	0.54 μM Compound: 1	Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B and MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B and MTT assay	[PMID: 22119124]
MDA-MB-231	IC₅₀	2.27 μM Compound: Diphyllin	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 38336655]
MIA PaCa-2	IC₅₀	0.271 μM Compound: 1	Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay Antiproliferative activity against human MIA PaCa-2 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay	[PMID: 36122549]
Monocyte	IC₅₀	35.2 μM Compound: Diphyllin	Antiproliferative activity against Homo sapiens (human) monocytes Antiproliferative activity against Homo sapiens (human) monocytes	10.1007/s00044-011-9937-1
MONO-MAC-6	IC₅₀	3.9 μM Compound: 1	Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
NCI-H838	IC₅₀	16 μM Compound: 1	Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
Panc02	IC₅₀	0.061 μM Compound: 1	Antiproliferative activity against mouse Panc02 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay Antiproliferative activity against mouse Panc02 cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay	[PMID: 36122549]
PaTu 8988t	IC₅₀	0.102 μM Compound: 1	Antiproliferative activity against human PaTu 8988t cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay Antiproliferative activity against human PaTu 8988t cells assessed as inhibition of cell proliferation incubated for 72 hrs by SRB assay	[PMID: 36122549]
PC-3	IC₅₀	0.21 μM Compound: Diphyllin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth after 72 hrs by MTT assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 38336655]
PC-3	IC₅₀	3.8 μM Compound: 1	Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
PC-3	IC₅₀	7.2 μM Compound: 6	Cytotoxicity against androgen-independent human PC3 cells Cytotoxicity against androgen-independent human PC3 cells	[PMID: 17256902]
Platelet	IC₅₀	>100 μM Compound: 10	Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method	[PMID: 8988600]
SW480	IC₅₀	3.4 μM Compound: 5a	Anticancer activity against Homo sapiens (human) SW480 cells assessed as decrease in cell growth after 3 days by MTT assay Anticancer activity against Homo sapiens (human) SW480 cells assessed as decrease in cell growth after 3 days by MTT assay	10.1007/s00044-012-0245-1
SW480	IC₅₀	3.4 μM Compound: Diphyllin	Cytotoxicity against Homo sapiens (human) SW480 cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) SW480 cells after 72 hr by MTT assay	10.1007/s00044-011-9937-1
TE-1	IC₅₀	0.28 μM Compound: 1	Antiproliferative activity against human TE-1 cells measured after 2 days by CCK-8 method Antiproliferative activity against human TE-1 cells measured after 2 days by CCK-8 method	[PMID: 36857518]
U-87MG ATCC	IC₅₀	26.1 μM Compound: 1	Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
Vero C1008	IC₅₀	>100 μM Compound: 1	Cytotoxicity against African green monkey Vero E6 cells incubated for 48 hrs by CCK-8 assay Cytotoxicity against African green monkey Vero E6 cells incubated for 48 hrs by CCK-8 assay	[PMID: 36857518]

In Vitro

Acid Influx Assay: Diphyllin (0.6 nM; 30 min) potently inhibits acid influx in isolated vesicles from bovine adrenal medullae^[2].
V-ATPase Assay: Diphyllin (17 nM; 1 h) inhibits V-ATPase activity^[2].
Osteoclast Acidification Assay: Diphyllin (1 μM; 45 min) dose-dependently inhibits lysosomal acidification in human osteoclasts, and completely abolishes the orange signal at 1 μM^[2].
Resorption Assays: Diphyllin (14 nM; 5 days) dose-dependently inhibits human osteoclastic bone resorption, while increasing cell viability, tartrate-resistant acid phosphatase (TRACP) activity, and the number of calcitonin receptor-positive cells; Diphyllin (49 nM; 5 days) inhibits calcium release from bone slices during osteoclastic bone resorption; Diphyllin (29 nM; 5 days) dose-dependently decreases the resorbed bone area^[2].
Osteoblastogenesis Assay: Diphyllin (up to 100 nM; 21 days) shows no effect on bone formation and no cytotoxicity in the mouse osteoblastic cell line MC3T3-E1^[2].
Macrophage-related Assays: In LPS/IFN-γ-activated murine peritoneal macrophages, Diphyllin (12.5 μM; 24 h) inhibits NO production; Diphyllin (12.5-50 μM; 24 h) inhibits the production of TNF-α and IL-12; when macrophages are pre-activated with LPS/IFN-γ for 24 h, Diphyllin (50 μM; 24 h) still inhibits NO production^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Human osteoclasts
Concentration:

1 nM-3 μM
Incubation Time:

5 days
Result:

Increased cell viability at concentrations lower than 3 μM. At 3 μM, cell viability, osteoclast number, and TRACP activity decreased.

Cell Cytotoxicity Assay^[2]

Cell Line:

Mouse osteoblastic cell line MC3T3-E1
Concentration:

10 nM-100 nM
Incubation Time:

21 days
Result:

Showed no cytotoxic effects on the osteoblasts up to 100 nM and had no effect on the bone formation induced by β-glycerol phosphate and ascorbic acid.

Western Blot Analysis^[3]

Cell Line:

Murine peritoneal macrophages
Concentration:

6.25 μM-100 μM
Incubation Time:

24 h
Result:

Dose-dependently inhibited the production of NO, TNF-α, and IL-12 in LPS/IFN-γ-activated murine peritoneal macrophages.
Inhibited NO productionm at 50 μM, in pre-activated macrophages with LPS/IFN-γ for 24 h.

In Vivo

Diphyllin (20 mg/kg; oral; once a day; for 10 consecutive days) significantly inhibits the tumor growth of SGC7901 cells in mice, reduced tumor weight, downregulated the expression of V-ATPase in tumor tissues, and reduced the levels of MMP-2 and MMP-9 in serum^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 22055-22-7
Appearance Solid
Molecular Weight 380.35
Formula C₂₁H₁₆O₇
Color Off-white to gray
SMILES

O=C1OCC2=C(O)C3=CC(OC)=C(OC)C=C3C(C4=CC=C(OCO5)C5=C4)=C12
Structure Classification
- Phenylpropanoids
- Lignans
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
bioRxiv

2025 Sep 30.
SSRN

2025 Aug 20.

Solvent & Solubility

In Vitro:

DMSO : 14.29 mg/mL (37.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6292 mL

13.1458 mL

26.2916 mL

5 mM

0.5258 mL

2.6292 mL

5.2583 mL

10 mM

0.2629 mL

1.3146 mL

2.6292 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.43 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.43 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg
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g
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μL
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xin-Ya Xu, et al. Anti-HIV Lignans From Justicia Procumbens. Chin J Nat Med. 2019 Dec;17 (12) :945-952. [Content Brief]

[2]. Mette G Sørensen, et al. Diphyllin, a Novel and Naturally Potent V-ATPase Inhibitor, Abrogates Acidification of the Osteoclastic Resorption Lacunae and Bone Resorption. J Bone Miner Res. 2007 Oct;22 (10) :1640-8. [Content Brief]

[3]. Yerra Koteswara Rao, et al. Anti-inflammatory Activities of Constituents Isolated From Phyllanthus Polyphyllus. J Ethnopharmacol. 2006 Jan 16;103 (2) :181-6. [Content Brief]

[4]. Shen W, et al. Effects of diphyllin as a novel V-ATPase inhibitor on gastric adenocarcinoma. Eur J Pharmacol. 2011 Sep 30;667 (1-3) :330-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6292 mL	13.1458 mL	26.2916 mL	65.7289 mL
	5 mM	0.5258 mL	2.6292 mL	5.2583 mL	13.1458 mL
	10 mM	0.2629 mL	1.3146 mL	2.6292 mL	6.5729 mL
	15 mM	0.1753 mL	0.8764 mL	1.7528 mL	4.3819 mL
	20 mM	0.1315 mL	0.6573 mL	1.3146 mL	3.2864 mL
	25 mM	0.1052 mL	0.5258 mL	1.0517 mL	2.6292 mL
	30 mM	0.0876 mL	0.4382 mL	0.8764 mL	2.1910 mL

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Diphyllin

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Publications Citing Use of MedChemExpress (MCE) Diphyllin

All HIV Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Diphyllin Related Classifications

HY-N2532 Related Classifications