1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. DJ001

DJ001 

Cat. No.: HY-133146 Purity: 99.59%
Handling Instructions

DJ001 is a highly specific, selective and non-competitive protein tyrosine phosphatase-σ (PTPσ) inhibitor with an IC50 of 1.43 μM. DJ001 displays no inhibitory activity against other phosphatases, with only modest inhibitory activity against Protein Phosphatase 5. DJ001 promotes promote hematopoietic stem cell regeneration.

For research use only. We do not sell to patients.

DJ001 Chemical Structure

DJ001 Chemical Structure

CAS No. : 2161305-12-8

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 385 In-stock
Estimated Time of Arrival: December 31
5 mg USD 350 In-stock
Estimated Time of Arrival: December 31
10 mg USD 550 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1650 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2250 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

DJ001 is a highly specific, selective and non-competitive protein tyrosine phosphatase-σ (PTPσ) inhibitor with an IC50 of 1.43 μM. DJ001 displays no inhibitory activity against other phosphatases, with only modest inhibitory activity against Protein Phosphatase 5. DJ001 promotes promote hematopoietic stem cell regeneration[1].

IC50 & Target

IC50: 1.43 μM (Protein tyrosine phosphatase-σ)[1]

In Vitro

DJ001 (5-1000 ng/mL; 3-7 days; BM KSL cells) treatment increases the percentages and numbers of BM KSL cells in culture compared with control cultures. DJ001 treatment also significantly increases the numbers of colony forming cells (CFCs) in 3 day culture of BM KSL cells[1].
The BM KSL cells are irradiated with 300 cGy and placed in media (containing 20 ng/mL Thrombopoietin, 100 ng/mL stem cell factor (SCF), 50 ng/mL Flt3 ligand, TSF) with and without 1 μg/mL DJ001 for 3 days. DJ001 treatment increases recovery of BM CFCs and multipotent colony-forming unit–granulocyte erythroid monocyte megakaryocyte (CFU-GEMM) colonies compared with control cultures[1].

Cell Viability Assay[1]

Cell Line: BM KSL cells
Concentration: 5 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL
Incubation Time: 3 days or 7 days
Result: Increased the percentages and numbers of BM KSL cells. Also significantly increased the numbers of colony forming cells (CFCs) in 3 day culture of BM KSL cells.
In Vivo

DJ001 (5 mg/kg; subcutaneous injection; for 24 hours; female C57BL/6 mice) significantly decreases the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24 h following 500 cGy TBI. DJ001 suppresses radiation-induced HSC apoptosis via activation of the RhoGTPase, RAC1, and induction of BCL-XL[1].

Animal Model: Female C57BL/6 mice (8-10-week-old) irradiated with total body irradiation (TBI)[1]
Dosage: 5 mg/kg
Administration: Subcutaneous injection; for 24 hours
Result: Significantly decreased the percentage of apoptotic BM KSL cells in C57BL/6 mice at 24 h following 500 cGy TBI.
Molecular Weight

268.27

Formula

C₁₅H₁₂N₂O₃

CAS No.

2161305-12-8

SMILES

O=C(C1=CC=CC=C1)/C=C\NC2=CC=CC([N+]([O-])=O)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (931.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7276 mL 18.6379 mL 37.2759 mL
5 mM 0.7455 mL 3.7276 mL 7.4552 mL
10 mM 0.3728 mL 1.8638 mL 3.7276 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

DJ001DJ 001DJ-001PhosphatasePTPσhematopoieticregenerationapoptosisnumbersHSCRhoGTPaseRAC1BCL-XLInhibitorinhibitorinhibit

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DJ001
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