Monochlorobimane
Based on 20 publication(s) in Google Scholar
Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 76421-73-3
- Formula: C10H11ClN2O2
- Molecular Weight:226.66
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Monochlorobimane
More- Nat Commun. 2025 Mar 20;16(1):2740. [Abstract]
- J Nanobiotechnology. 2026 Feb 13;24(1):235. [Abstract]
- Biomaterials. 2025 Feb 12:318:123188. [Abstract]
- Cell Death Dis. 2026 Feb 23;17(1):243. [Abstract]
- Int J Biol Sci. 2022 Jul 11;18(11):4545-4559. [Abstract]
- Antioxidants (Basel). 2024 Jun 29;13(7):798. [Abstract]
- Free Radic Biol Med. 2026 Sep:253:785-802. [Abstract]
- Ecotoxicol Environ Saf. 2025 Jun 4:300:118461. [Abstract]
- Int J Mol Sci. 2023 Jul 28;24(15):12104. [Abstract]
- FASEB J. 2025 Jul 31;39(14):e70846. [Abstract]
- Curr Issues Mol Biol. 2024 Jun 11;46(6):5812-5824. [Abstract]
- J Cell Physiol. 2025 Jan;240(1):e31494. [Abstract]
- Exp Cell Res. 2026 May 14:115062. [Abstract]
- Food Chem Toxicol. 2023 Jun:176:113785. [Abstract]
- Anim Reprod Sci. 2026 May 5;292:108229.
- Reprod Biol. 2025 Nov 14;26(1):101102. [Abstract]
- Res Sq. 2026 Mar 4.
- SSRN. 2025 Aug 7.
- bioRxiv. 2025 March 17.
- Research Square Preprint. 2024 Feb 5.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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IF
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add Monochlorobimane working solution and incubate at 37°C for 30 minutes (optimized according to the experiment).
2.3 The fluorescence intensity is measured with fluorescence microscopy or Fluorescence microplate reader (Ex/Em = 380/480 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 76421-73-3
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Appearance Solid
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Molecular Weight 226.66
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Formula C10H11ClN2O2
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Color Light yellow to yellow
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SMILES
O=C1N(C(C(C)=C2C)=O)N2C(CCl)=C1C
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Synonyms
Chlorobimane，mBBr
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (20)
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Journal Impact Factor
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Most Recent
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Nat Commun
Integrative single-cell metabolomics and phenotypic profiling reveals metabolic heterogeneity of cellular oxidation and senescence. [Abstract]2025 Mar 20;16(1):2740. PMID: 40113759 -
J Nanobiotechnology
Bioactive manganese oxide nanoassembly as a highly sensitive magnetic resonance imaging probe for grading diagnosis of liver injury. [Abstract]2026 Feb 13;24(1):235. PMID: 41689006 -
Biomaterials
2025 Feb 12:318:123188. PMID: 39954312
Monochlorobimane purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Feb 12:318:123188. [Abstract]
Monochlorobimane (40 μM, 37°C, 20 min) was used to measure GSH levels in HepG2 cells.
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Cell Death Dis
PDHA1-acetylation signaling suppresses cuproptosis to attenuate anti-androgen effect in prostate cancer. [Abstract]2026 Feb 23;17(1):243. PMID: 41730841 -
Int J Biol Sci
Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling. [Abstract]2022 Jul 11;18(11):4545-4559. PMID: 35864954
Monochlorobimane purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jul 11;18(11):4545-4559. [Abstract]
Intracellular GSH levels in the retinal tissues of EAU mice were quantified using monochlorobimane (30 min).
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Antioxidants (Basel)
Design and Synthesis of Novel Antioxidant 2-Substituted-5,7,8-Trimethyl-1,4-Benzoxazine Hybrids: Effects on Young and Senescent Fibroblasts. [Abstract]2024 Jun 29;13(7):798. PMID: 39061867
Monochlorobimane purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Jun 29;13(7):798. [Abstract]
Monochlorobimane (mCB, 5 μM, diluted in Hanks' Balanced Salt Solution (HBSS) for 4 h) was used to assess glutathione (GSH) levels in human skin fibroblasts.
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Free Radic Biol Med
Bushen Tiaojing decoction ameliorates meiotic impairment to improve oocyte quality during aging through blocking the iron overload-induced p53/CDK1/ESPL1 pathway. [Abstract]2026 Sep:253:785-802. PMID: 42214643 -
Ecotoxicol Environ Saf
Microplastics (MPs) exposure impairs porcine oocyte quality by triggering oxidative stress-directed DNA damage and apoptosis with metabolomic alterations. [Abstract]2025 Jun 4:300:118461. PMID: 40472692
Monochlorobimane purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Jun 4:300:118461. [Abstract]
Monochlorobimane (20–100 μM, 2 h) was used to determine intracellular GSH levels in oocytes.
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Int J Mol Sci
Biological Activities of Ceratonia siliqua Pod and Seed Extracts: A Comparative Analysis of Two Cretan Cultivars. [Abstract]2023 Jul 28;24(15):12104. PMID: 37569477
Monochlorobimane purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Jul 28;24(15):12104. [Abstract]
MCB (5 μM, diluted in Hanks' balanced salt solution (HBSS) for 4 h) was used to assess glutathione (GSH) levels in human skin fibroblasts.
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FASEB J
Reverse Effects of Nicotinamide Mononucleotide Supplementation on Declining Quality of Oocytes With Polycystic Ovary Syndrome. [Abstract]2025 Jul 31;39(14):e70846. PMID: 40678956 -
Curr Issues Mol Biol
Biological Activities of Citrus-Derived Extracellular Vesicles on Human Cells: The Role of Preservation. [Abstract]2024 Jun 11;46(6):5812-5824. PMID: 38921018 -
J Cell Physiol
2025 Jan;240(1):e31494. PMID: 39639679 -
Exp Cell Res
Glutathione decline coordinates mitochondria to license histone acetylation and zygotic genome activation. [Abstract]2026 May 14:115062. PMID: 42140305 -
Food Chem Toxicol
An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. [Abstract]2023 Jun:176:113785. PMID: 37080529 -
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Reprod Biol
Phloretin enhances the efficiency of sheep oocyte IVM and early embryonic development by bolstering antioxidant defenses, inhibiting apoptosis, and promoting development. [Abstract]2025 Nov 14;26(1):101102. PMID: 41240406 -
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Solvent & Solubility
DMSO : 50 mg/mL (220.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Monochlorobimane (mCB) is added to the second half liver tissue homogenate to a final concentration of 100 mM along with glutathione S-transferase (1 U/mL) obtained from equine liver; the homogenate is then allowed to incubate at room temperature for 30 min. The Glutathione (GSH)-Monochlorobimane adduct is measured in a microtiter reader[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (453 KB)
- English - EN (453 KB)
- Français - FR (453 KB)
- Deutsch - DE (453 KB)
- Norwegian - NO (453 KB)
- Español - ES (453 KB)
- Swedish - SV (453 KB)
- Italian - IT (453 KB)
- Korean - KR (453 KB)
- Portuguese - PT (453 KB)
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Handling Instructions (2659 KB)
References
[1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7. [Content Brief]
[3]. Fotopoulou T, et al. Design and Synthesis of Novel Antioxidant 2-Substituted-5,7,8-Trimethyl-1,4-Benzoxazine Hybrids: Effects on Young and Senescent Fibroblasts. Antioxidants (Basel). 2024 Jun 29;13(7):798. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.4119 mL | 22.0595 mL | 44.1189 mL | 110.2974 mL |
| 5 mM | 0.8824 mL | 4.4119 mL | 8.8238 mL | 22.0595 mL | |
| 10 mM | 0.4412 mL | 2.2059 mL | 4.4119 mL | 11.0297 mL | |
| 15 mM | 0.2941 mL | 1.4706 mL | 2.9413 mL | 7.3532 mL | |
| 20 mM | 0.2206 mL | 1.1030 mL | 2.2059 mL | 5.5149 mL | |
| 25 mM | 0.1765 mL | 0.8824 mL | 1.7648 mL | 4.4119 mL | |
| 30 mM | 0.1471 mL | 0.7353 mL | 1.4706 mL | 3.6766 mL | |
| 40 mM | 0.1103 mL | 0.5515 mL | 1.1030 mL | 2.7574 mL | |
| 50 mM | 0.0882 mL | 0.4412 mL | 0.8824 mL | 2.2059 mL | |
| 60 mM | 0.0735 mL | 0.3677 mL | 0.7353 mL | 1.8383 mL | |
| 80 mM | 0.0551 mL | 0.2757 mL | 0.5515 mL | 1.3787 mL | |
| 100 mM | 0.0441 mL | 0.2206 mL | 0.4412 mL | 1.1030 mL |
Monochlorobimane Related Classifications
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