Grisnilimab
Based on 1 Customer Validation
Grisnilimab (WT1) is an IgG2a monoclonal antibody targeting CD7. Grisnilimab only binds to lymphoid tissues and T lymphocytes, with no off-target binding to normal tissues. Grisnilimab can be used to synthesize the immunotoxin WT1-SMPT-dgRTA, which exerts cytotoxic effects on T-lymphoblastic leukemia cells. Grisnilimab is applicable to relevant research on leukemia.
For research use only. We do not sell to patients.
- Purity : 99.43%
- CAS No.: 2367001-70-3
- Molecular Weight:146.39 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Mouse IgG2a
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD7
In Vitro
Grisnilimab (2.5 μg/mL) specifically binds to human lymphoid tissues (T zone pattern) and scattered T lymphocytes, and shows no binding activity to other tested normal human tissues[1].
Grisnilimab (3 μL of 1 mg/mL solution; 30 min) binds to the CD7 antigen on the surface of peripheral blood mononuclear cells from rhesus monkeys[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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IgG2a-lambda
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized Human CD7 Protein (His Tag) can bind Grisnilimab. The EC50 for this effect is 22.65 ng/mL.
Chemical Information
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CAS No. 2367001-70-3
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Appearance Liquid
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Molecular Weight 146.39 kDa
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Color Colorless to light yellow
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SMILES
[Grisnilimab]
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Synonyms
WT1
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (256 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Grisnilimab Related Classifications
HY-P99650 Related Classifications
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