ZA3-Ep10
Based on 1 Customer Validation
ZA3-Ep10 is a zwitterionic lipid used in lipid nanoparticles formulation for in vivo RNA delivery.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 2090299-48-0
- Formula: C66H138N6O9S
- Molecular Weight:1191.90
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
ZA3-Ep10 Cas9 mRNA ZNPs (4 h) shows high Cas9 mRNA level in A549 lung cancer cells[4].
ZNPs formulated with the lead ZA3-EP10, are capable of delivering different RNA cargoes including sgRNA, mCherry mRNA, and luciferase mRNA to human ovarian cancer cell line IGROV1[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2090299-48-0
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Appearance Oil
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Molecular Weight 1191.90
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Formula C66H138N6O9S
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Color White to light yellow
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SMILES
O=C(CCN(CC(CCCCCCCC)O)CCN(CCN(CC(CCCCCCCC)O)CC(CCCCCCCC)O)CCN(CC(CCCCCCCC)O)CC(CCCCCCCC)O)NCC[N+](C)(CCCS(=O)([O-])=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (83.90 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Cationic sulfonamide amino lipids and amphiphilic zwitterionic amino lipids. WO2017201076A1
[2]. Melamed JR, Hajj KA, Chaudhary N, et al. Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery. J Control Release. 2022;341:206-214. [Content Brief]
[3]. Melamed JR, et al. Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery. J Control Release. 2022 Jan;341:206-214. [Content Brief]
[4]. Miller JB, et al. Non-Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co-Delivery of Cas9 mRNA and sgRNA. Angew Chem Int Ed Engl. 2017 Jan 19;56(4):1059-1063. [Content Brief]
[5]. He S, et al. Zwitterionic materials for nucleic acid delivery and therapeutic applications. J Control Release. 2024 Jan;365:919-935. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8390 mL | 4.1950 mL | 8.3900 mL | 20.9749 mL |
| 5 mM | 0.1678 mL | 0.8390 mL | 1.6780 mL | 4.1950 mL | |
| 10 mM | 0.0839 mL | 0.4195 mL | 0.8390 mL | 2.0975 mL | |
| 15 mM | 0.0559 mL | 0.2797 mL | 0.5593 mL | 1.3983 mL | |
| 20 mM | 0.0419 mL | 0.2097 mL | 0.4195 mL | 1.0487 mL | |
| 25 mM | 0.0336 mL | 0.1678 mL | 0.3356 mL | 0.8390 mL | |
| 30 mM | 0.0280 mL | 0.1398 mL | 0.2797 mL | 0.6992 mL | |
| 40 mM | 0.0210 mL | 0.1049 mL | 0.2097 mL | 0.5244 mL | |
| 50 mM | 0.0168 mL | 0.0839 mL | 0.1678 mL | 0.4195 mL | |
| 60 mM | 0.0140 mL | 0.0699 mL | 0.1398 mL | 0.3496 mL | |
| 80 mM | 0.0105 mL | 0.0524 mL | 0.1049 mL | 0.2622 mL |
ZA3-Ep10 Related Classifications
HY-145794 Related Classifications
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