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  3. Fluorescent Dye
  4. DMN-Tre

DMN-Tre is a conjugate of a solvatochromic fluorescent dye and trehalose. DMN-Tre takes advantage of the substrate promiscuity of the endogenous antigen 85 protein complex in mycobacteria to be metabolically integrated into the hydrophobic mycobacterial membrane. Once entering this hydrophobic environment, the linked DMN dye fluorescence is "turned on", enabling specific labeling. DMN-Tre can be used to reflect bacterial metabolic activity and support physiological studies of Mycobacterium tuberculosis.

For research use only. We do not sell to patients.

DMN-Tre

DMN-Tre Chemical Structure

CAS No. : 2177287-88-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
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10 mg In-stock
25 mg In-stock
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DMN-Tre Related Antibodies

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Description

DMN-Tre is a conjugate of a solvatochromic fluorescent dye and trehalose. DMN-Tre takes advantage of the substrate promiscuity of the endogenous antigen 85 protein complex in mycobacteria to be metabolically integrated into the hydrophobic mycobacterial membrane. Once entering this hydrophobic environment, the linked DMN dye fluorescence is "turned on", enabling specific labeling. DMN-Tre can be used to reflect bacterial metabolic activity and support physiological studies of Mycobacterium tuberculosis[1][2].

In Vitro

Guide (The following are our recommended solutions. These are only Guide and should be modified according to your specific needs).
1.Preparation: Prepare or obtain DMN-Tre stock solution.
2. Incubation: Incubate samples with a final concentration of 100
M (for M. smegmatis) or up to 1 mM (for M. tuberculosis (Mtb)) of DMN-Tre.
3. Time: For M. smegmatis, 30 min is typical. For Mtb in sputum or cultures, labeling may take up to 16 hours or overnight.
4. Detection/Fixation: The cells can be imaged directly. For clinical sputum samples, this is followed by fixation (e.g., 4% formaldehyde or 2.5% glutaraldehyde) before analysis with a fluorescent microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DMN-Tre Related Antibodies
Molecular Weight

564.54

Formula

C26H32N2O12
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1C2=CC=C(N(C)C)C3=C2C(C(N1C[C@H]4O[C@@H]([C@@H]([C@H]([C@@H]4O)O)O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)CO)=O)=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 20 mg/mL (35.43 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7714 mL 8.8568 mL 17.7135 mL
5 mM 0.3543 mL 1.7714 mL 3.5427 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.7714 mL 8.8568 mL 17.7135 mL 44.2838 mL
5 mM 0.3543 mL 1.7714 mL 3.5427 mL 8.8568 mL
10 mM 0.1771 mL 0.8857 mL 1.7714 mL 4.4284 mL
15 mM 0.1181 mL 0.5905 mL 1.1809 mL 2.9523 mL
20 mM 0.0886 mL 0.4428 mL 0.8857 mL 2.2142 mL
25 mM 0.0709 mL 0.3543 mL 0.7085 mL 1.7714 mL
30 mM 0.0590 mL 0.2952 mL 0.5905 mL 1.4761 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DMN-Tre Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMN-Tre2177287-88-4Fluorescent DyeAg85 enzymeCorynebacterium glutamicumActinobacteriamycobacterial cell wallscorynebacterial cell wallsMycobacterium smegmatisTHP-1 human monocyte-derived macrophagesMycobacterium tuberculosisM. bovis BCGMycobacterium marinumInhibitorinhibitorinhibit

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