2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK
  3. Reactive Oxygen Species ROS Kinase
  4. DMNQ

DMNQ is a redox cycling agent. DMNQ produces hydrogen peroxide in cells in a concentration-dependent manner. DMNQ can induce the increase of ROS production.

For research use only. We do not sell to patients.

DMNQ Chemical Structure

DMNQ Chemical Structure

CAS No. : 6956-96-3

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Based on 5 publication(s) in Google Scholar

DMNQ Related Antibodies

Top Publications Citing Use of Products

    DMNQ purchased from MedChemExpress. Usage Cited in: Mediat Inflamm. 2020 Jun 6;2020:4321912.  [Abstract]

    qPCR shows that the levels of IL-6, IL-1β, and TNF-α in DMNQ group increased compared with the M5580 group, indicating that DMNQ could weaken the inhibitory effect of M5580 on inflammation production.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DMNQ is a redox cycling agent. DMNQ produces hydrogen peroxide in cells in a concentration-dependent manner. DMNQ can induce the increase of ROS production[1][2].

    IC50 & Target

    γ-glutamylcysteine synthetase (γ-GCs)[2].

    In Vitro

    DMNQ (20-100 μM; 30 min) can induce H2O2 production in a concentration-dependent manner in A549-S cells[1].
    DMNQ (5-10 μM; 0-24 h) can increase glutathione (GSH) (physiological antioxidant) content in L2 cells in a time- and dose-dependent manner by enhancing the activity of γ-glutamylcysteine synthetase (γ-GCs) (70%) [2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DMNQ Related Antibodies

    Western Blot Analysis[2]

    Cell Line: L2
    Concentration: 5 μM, 10 μM
    Incubation Time: 24 h
    Result: Increased the level of γGCS-HS protein.
    Molecular Weight

    218.21

    Formula

    C12H10O4
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C(OC)=C(C(C2=CC=CC=C21)=O)OC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (229.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5827 mL 22.9137 mL 45.8274 mL
    5 mM 0.9165 mL 4.5827 mL 9.1655 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (11.46 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (11.46 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.26%

    COA (253 KB) LCMS (258 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.5827 mL 22.9137 mL 45.8274 mL 114.5685 mL
    5 mM 0.9165 mL 4.5827 mL 9.1655 mL 22.9137 mL
    10 mM 0.4583 mL 2.2914 mL 4.5827 mL 11.4569 mL
    15 mM 0.3055 mL 1.5276 mL 3.0552 mL 7.6379 mL
    20 mM 0.2291 mL 1.1457 mL 2.2914 mL 5.7284 mL
    25 mM 0.1833 mL 0.9165 mL 1.8331 mL 4.5827 mL
    30 mM 0.1528 mL 0.7638 mL 1.5276 mL 3.8190 mL
    40 mM 0.1146 mL 0.5728 mL 1.1457 mL 2.8642 mL
    50 mM 0.0917 mL 0.4583 mL 0.9165 mL 2.2914 mL
    60 mM 0.0764 mL 0.3819 mL 0.7638 mL 1.9095 mL
    80 mM 0.0573 mL 0.2864 mL 0.5728 mL 1.4321 mL
    100 mM 0.0458 mL 0.2291 mL 0.4583 mL 1.1457 mL
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    DMNQ Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DMNQ6956-96-3Reactive Oxygen SpeciesROS KinaseOxidative stressInhibitorinhibitorinhibit

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