2. Cell Cycle/DNA Damage Cytoskeleton Antibody-drug Conjugate/ADC Related Apoptosis
  3. Microtubule/Tubulin ADC Payload Apoptosis
  4. Dolastatin 15

Dolastatin 15 (DLS 15), a depsipeptide derived from Dolabella auricularia, is a potent antimitotic agent structurally related to the antitubulin agent Dolastatin 10. Dolastatin 15 induces cell cycle arrest and apoptosis in multiple myeloma cells. Dolastatin 15 can be used as an ADC cytotoxin.

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Custom Peptide Synthesis

Dolastatin 15

Dolastatin 15 Chemical Structure

CAS No. : 123884-00-4

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Dolastatin 15 Related Antibodies

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Description

Dolastatin 15 (DLS 15), a depsipeptide derived from Dolabella auricularia, is a potent antimitotic agent structurally related to the antitubulin agent Dolastatin 10. Dolastatin 15 induces cell cycle arrest and apoptosis in multiple myeloma cells. Dolastatin 15 can be used as an ADC cytotoxin[1][2][3].

IC50 & Target

Auristatin

 

Cellular Effect
Cell Line Type Value Description References
CTLL IC50
3 nM
Compound: 1 (4S,7S)(Dolastatin 15)
In vitro interleukin-2-dependent proliferation of the murine cytotoxlc T cell line (CTLL) was determined
In vitro interleukin-2-dependent proliferation of the murine cytotoxlc T cell line (CTLL) was determined
10.1016/S0960-894X(01)80540-0
HT-29 IC50
0.29 nM
Compound: dolastatin 15
Inhibitory activity against growth of HT-29 cell line
Inhibitory activity against growth of HT-29 cell line
[PMID: 9554885]
Jurkat IC50
5 nM
Compound: 1 (4S,7S)(Dolastatin 15)
In vitro growth factor-independent proliferation against human leukemia T cell line jurkat was determined
In vitro growth factor-independent proliferation against human leukemia T cell line jurkat was determined
10.1016/S0960-894X(01)80540-0
L1210 IC50
0.13 nM
Compound: dolastatin 15
Inhibitory activity against growth of L1210 cell line
Inhibitory activity against growth of L1210 cell line
[PMID: 9554885]
L1210 IC50
0.15 nM
Compound: 1 (4S,7S)(Dolastatin 15)
In vitro growth factor-independent proliferation against murine leukemia L1210 was determined
In vitro growth factor-independent proliferation against murine leukemia L1210 was determined
10.1016/S0960-894X(01)80540-0
Lymphoma cell line IC50
0.012 nM
Compound: 1 (4S,7S)(Dolastatin 15)
Antiproliferative effect against human lymphoma cells was determined; (0.012 to 0.12 nM)
Antiproliferative effect against human lymphoma cells was determined; (0.012 to 0.12 nM)
10.1016/S0960-894X(01)80540-0
MCF7 IC50
0.04 nM
Compound: dolastatin 15
Inhibitory activity against growth of MCF-7 cell line
Inhibitory activity against growth of MCF-7 cell line
[PMID: 9554885]
Osteoclast-like IC50
17 nM
Compound: 1 (4S,7S)(Dolastatin 15)
In vitro growth factor-dependent proliferation against murine bone marrow cell was determined
In vitro growth factor-dependent proliferation against murine bone marrow cell was determined
10.1016/S0960-894X(01)80540-0
P388 ED50
2.4 ng/mL
Compound: Dolastatin15
Anticancer activity against lymphocytic leukemia P388 cells was determined
Anticancer activity against lymphocytic leukemia P388 cells was determined
[PMID: 9871690]
Panel leukemia (Carcinoma cell lines) IC50
0.1 nM
Compound: 1 (4S,7S)(Dolastatin 15)
Antiproliferative effect against continuous human leukemia cell lines and peripheral blood cells from patients with acute myeloid leukemia was determined; (0.1 to 1 nM)
Antiproliferative effect against continuous human leukemia cell lines and peripheral blood cells from patients with acute myeloid leukemia was determined; (0.1 to 1 nM)
10.1016/S0960-894X(01)80540-0
In Vitro

Dolastatin 15 (DLS 15) induces cell cycle arrest at the G2/M phase followed by apoptosis in various human myeloma cell lines (RPMI8226, U266, and IM9). Dolastatin 15 induces apoptosis of myeloma cells via activation of both mitochondrial- and Fas (CD95)/Fas-L (CD95-L)-mediated pathways[2]. Dolastatin 15 (DLS 15) displays growth inhibitory activity against all four SCLC cell lines (NCI-H69, NCI-H82, NCI-H345, NCI-H446) with IC50 values ranging from 0.039-28.8 nM, which were 2.7-9.2-fold higher than the values for dolastatin 10. All four SCLC cell lines underwent G2/M arrest within 24 hours of exposure to dolastatin 15[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dolastatin 15 Related Antibodies
In Vivo

Dolastatin 15 conjugates to Trastuzumab via lysine residues at the drug C-terminus using a non-cleavable linker (Trastuzumab-amide-C-term-Dol15) produced target-dependent growth inhibition of cells endogenously expressing high HER2 levels (i.e., SK-BR-3, SK-OV-3) in vitro. This ADC was effective at varying doses (i.e., 10 and 20 mg/kg) in the SK-OV-3 human ovarian cancer xenograft[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

837.06

Formula

C45H68N6O9
CAS No.
Shipping

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Dolastatin 15 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dolastatin 15123884-00-4DLS 15Dolastatin15Dolastatin-15DLS15DLS-15Microtubule/TubulinADC PayloadApoptosisADC Cytotoxindepsipeptidecyclearrestantitubulin multiplemyelomacells cytotoxicmicrotubuleInhibitorinhibitorinhibit

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