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  4. DSP Crosslinker

DSP Crosslinker is an amine-reactive and cleavable protein crosslinker. DSP Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

For research use only. We do not sell to patients.

CAS No. : 57757-57-0

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of DSP Crosslinker:

DSP Crosslinker Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE DSP Crosslinker

IP

    DSP Crosslinker purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Oct 28.

    Co-immunoprecipitation of DOR and CNIH1 in DOR-RUSH HeLa cells at 0, 1, and 24 h after biotin addition using GFP-Trap beads. Interaction was detected in the presence of the DSP crosslinker (2 mM, 2 h), tubulin serves as a negative control. Quantification of CNIH1 co-precipitation signals at different time points is shown normalized to the 0 h time point. Significance was determined by one sample t-test. *, P < 0.05.

    DSP Crosslinker purchased from MedChemExpress. Usage Cited in: BMC Biol. 2021 Sep 7;19(1):194.  [Abstract]

    Effect of the co-expression of KDEL-cargo (hGHGFP-myc-KDEL) on KDELR1-mCherry co-immunoprecipitation in HeLa cells. HeLa cells co-transfected KDELR1-mCherry with hGH-GFP-myc or hGH-GFPmyc-KDEL were firstly treated with DSP crosslinker (2 mM) in PBS at 37 °C for 30 min. Cells were lysed and the extracts were immunoprecipitated with anti-RFP agarose beads.

    • Biological Activity

    • Purity & Documentation

    • Customer Review

    Description

    DSP Crosslinker is an amine-reactive and cleavable protein crosslinker. DSP Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

    Molecular Weight

    404.42

    Formula

    C14H16N2O8S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(CCSSCCC(ON1C(CCC1=O)=O)=O)ON2C(CCC2=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (123.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4727 mL 12.3634 mL 24.7268 mL
    5 mM 0.4945 mL 2.4727 mL 4.9454 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.73%

    SDS (394 KB)

    COA (248 KB) HNMR (122 KB) LCMS (103 KB)

    Handling Instructions (2659 KB)

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4727 mL 12.3634 mL 24.7268 mL 61.8169 mL
    5 mM 0.4945 mL 2.4727 mL 4.9454 mL 12.3634 mL
    10 mM 0.2473 mL 1.2363 mL 2.4727 mL 6.1817 mL
    15 mM 0.1648 mL 0.8242 mL 1.6485 mL 4.1211 mL
    20 mM 0.1236 mL 0.6182 mL 1.2363 mL 3.0908 mL
    25 mM 0.0989 mL 0.4945 mL 0.9891 mL 2.4727 mL
    30 mM 0.0824 mL 0.4121 mL 0.8242 mL 2.0606 mL
    40 mM 0.0618 mL 0.3091 mL 0.6182 mL 1.5454 mL
    50 mM 0.0495 mL 0.2473 mL 0.4945 mL 1.2363 mL
    60 mM 0.0412 mL 0.2061 mL 0.4121 mL 1.0303 mL
    80 mM 0.0309 mL 0.1545 mL 0.3091 mL 0.7727 mL
    100 mM 0.0247 mL 0.1236 mL 0.2473 mL 0.6182 mL
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    DSP Crosslinker Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DSP Crosslinker57757-57-0Biochemical Assay ReagentscleavableADClinkerInhibitorinhibitorinhibit

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