DSPE-PEG2000-TCO 

DSPE-PEG2000-TCO is a click chemistry coupling partner. DSPE-PEG2000-TCO undergoes a reverse electron-demand Diels-Alder click reaction to form a covalent linkage with tetrazine-modified Cetuximab (HY-P9905). DSPE-PEG2000-TCO maintains the stability of lipid nanoparticles (LNPs) and has high mRNA encapsulation efficiency, which supports cellular uptake and mRNA transfection. DSPE-PEG2000-TCO is used to prepare EGFR-targeted and APN-targeted mRNA-loaded LNPs, mediating receptor-dependent endocytosis, mRNA delivery, and intestinal epithelial transcytosis^[1][2].

DSPE-PEG2000-TCO forms micelles with Cetuximab (HY-P9905) that modify LNPs to mediate EGFR-dependent, near-complete (97-99%) transfection of HeLa cells with GFP mRNA, achieving 3-fold higher MFI than untargeted controls, with dose-dependent increases in intracellular mRNA payload and sensitivity to soluble Cetuximab inhibition^[1].
LNPs containing DSPE-PEG₂₀₀₀-TCO, functionalized with αAPN-VHH, are transcytosed across the porcine intestinal epithelial barrier in apical-out enteroids, with mRNA delivered to both epithelial and subepithelial compartments resulting in GFP expression^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DSPE-PEG2000-TCO-containing αAPN-VHH-LNPs enables efficient uptake by intestinal epithelial and subepithelial cells, mRNA translation, and transport to draining mesenteric lymph nodes in piglet gut loops^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2000 (Average)

(C₂H₄O)nC₅₃H₉₉N₂O₁₂P

1480940-53-1

Solid

White to off-white

O=C(OCCOCCNC(OC1CCC/C=C/CC1)=O)NCCOP(O)(OC[C@H](OC(CCCCCCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCCCCCC)=O)=O.[n]

Room temperature in continental US; may vary elsewhere.

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

• [1]. Patel J, et al. Preparation of Targeted Lipid Nanoparticles by Insertion of Either Tetrazine-trans-Cyclooctene or Thiol-Maleimide Micelles. Bioconjug Chem. 2026;37(2):406-417.  [Content Brief]

  [2]. Chen L, et al. Cell-specific mRNA delivery via nanobody-functionalized lipid nanoparticles. J Control Release. 2025;388(Pt 2):114365.  [Content Brief]