(E)-Antibacterial agent 224
Based on 1 Customer Validation
(E)-Antibacterial agent 224 is an isomer of Antibacterial agent 224 (HY-161752). Antibacterial agent 224 is a potent and selective synthetic hydrazone inhibitor against the Salmonella PhoP/PhoQ system.
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- CAS No.: 84889-00-9
- Formule: C12H10N2OS
- Masse moléculaire:230.29
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Antibacterial agent 224 (compound A16B1) represses the Salmonella PhoP/PhoQ system activity, specifically targeting PhoQ within the second transmembrane region. Antibacterial agent 224 demonstrates synergy with the antimicrobial peptide colistin, reduces the intramacrophage proliferation of Salmonella without being cytotoxic and enhances the survival of G. mellonella larvae systemically infected with Salmonella[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 84889-00-9
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Appearance Solid
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Masse moléculaire 230.29
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Formule C12H10N2OS
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SMILES
O=C(C1=CC=CC=C1)N/N=C/C2=CC=CS2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (434.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3424 mL | 21.7118 mL | 43.4235 mL | 108.5588 mL |
| 5 mM | 0.8685 mL | 4.3424 mL | 8.6847 mL | 21.7118 mL | |
| 10 mM | 0.4342 mL | 2.1712 mL | 4.3424 mL | 10.8559 mL | |
| 15 mM | 0.2895 mL | 1.4475 mL | 2.8949 mL | 7.2373 mL | |
| 20 mM | 0.2171 mL | 1.0856 mL | 2.1712 mL | 5.4279 mL | |
| 25 mM | 0.1737 mL | 0.8685 mL | 1.7369 mL | 4.3424 mL | |
| 30 mM | 0.1447 mL | 0.7237 mL | 1.4475 mL | 3.6186 mL | |
| 40 mM | 0.1086 mL | 0.5428 mL | 1.0856 mL | 2.7140 mL | |
| 50 mM | 0.0868 mL | 0.4342 mL | 0.8685 mL | 2.1712 mL | |
| 60 mM | 0.0724 mL | 0.3619 mL | 0.7237 mL | 1.8093 mL | |
| 80 mM | 0.0543 mL | 0.2714 mL | 0.5428 mL | 1.3570 mL | |
| 100 mM | 0.0434 mL | 0.2171 mL | 0.4342 mL | 1.0856 mL |
(E)-Antibacterial agent 224 Related Classifications
HY-161752A Related Classifications
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