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E235 is an expression regulator of activates transcription factor 4 (ATF4). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research.

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E235 Chemical Structure

E235 Chemical Structure

CAS No. : 891894-69-2

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Based on 1 publication(s) in Google Scholar

E235 Related Antibodies

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  • Purity & Documentation

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Description

E235 is an expression regulator of activates transcription factor 4 (ATF4). E235 reduces cell viability by activating integrated stress response (ISR) and DNA damage response signals. E235 has anti-proliferative activity and can be used for tumor research[1].

IC50 & Target

Activates transcription factor 4, ATF4[1]
In Vitro

E235 (1 µM; 2, 4, 8, 16 or 24 h) down-regulates the level of XBP-1s mRNA in HT1080 cells with time dependent manner[1].
E235 (0.1-10 µM; 4 d or 5 d) has anti-proliferative activity on HT1080, RPMI-8226, B16F10, 4T1, HT1080 shNT and HT1080 shATF4 cells[1].
E235 (0, 1, 5 and 10 µM; 4 h) increases the expression of p53 with dose-dependent manner in AG1522 cells[1].
E235 (1 µM; 2 h) increases the expression of p-Chk2 in AG1522 cells[1].
E235 (0, 1, 5 and 10 µM; 2 h) increases the expression of p-p53 in AG1522 and HT1080 cells[1].
E235 (0, 0.5, 1, 5 and 10 µM; 0.5 h) increases the expression of γ-H2AX with dose-dependent manner in HT1080 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

E235 Related Antibodies

Western Blot Analysis[1]

Cell Line: HT1080, B16F10 and AG1522 cells.
Concentration: 0, 1, 5 and 10 µM.
Incubation Time: 4 h.
Result: Increased the expression of ATF4 with dose-dependent manner.

Western Blot Analysis[1]

Cell Line: HT1080 cells.
Concentration: 0, 0.5, 1, 5 or 10 µM.
Incubation Time: 2, 4 or 8 h.
Result: Increased the expression of p-elF2α with time and dose dependent manner.

Cell Proliferation Assay[1]

Cell Line: HT1080 or RPMI-8226 cells.
Concentration: 1 µM.
Incubation Time: 0, 8, 16 and 24 h.
Result: Exhibited anti-proliferative activity.

Cell Viability Assay[1]

Cell Line: AG1522 cells.
Concentration: 0, 0.1, 0.5, 1 and 10 µM.
Incubation Time: 4 d.
Result: Inhibited cell viability with dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: HT1080 cells and B16F10 cells.
Concentration: 1 µM.
Incubation Time: 8, 16 and 24 h.
Result: Caused cell arrest during G2/M phase.
Molecular Weight

484.59

Formula

C28H25FN4OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C2N3C(SC2=C1)=NC(C4=CC=C(F)C=C4)=C3)NC5CCN(CC6=CC=CC=C6)CC5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (20.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0636 mL 10.3180 mL 20.6360 mL
5 mM 0.4127 mL 2.0636 mL 4.1272 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 1 mg/mL (2.06 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0636 mL 10.3180 mL 20.6360 mL 51.5900 mL
5 mM 0.4127 mL 2.0636 mL 4.1272 mL 10.3180 mL
10 mM 0.2064 mL 1.0318 mL 2.0636 mL 5.1590 mL
15 mM 0.1376 mL 0.6879 mL 1.3757 mL 3.4393 mL
20 mM 0.1032 mL 0.5159 mL 1.0318 mL 2.5795 mL
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E235 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

E235891894-69-2E 235E-235OthersHT1080 cellsB16F10 cellsAG1522 cellsp53p-Chk2p-p53γ-H2AXInhibitorinhibitorinhibit

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