2. GPCR/G Protein Neuronal Signaling
  3. Neurokinin Receptor
  4. EB1002

EB1002 is a highly selective, long-acting NK2R agonist. EB1002 exerts central appetite suppression, increases peripheral energy expenditure and enhances insulin sensitivity, which effectively reduces body weight, improves glucose and lipid metabolism, with favorable safety profiles. EB1002 can be used for research on diseases such as obesity and type 2 diabetes.

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Custom Peptide Synthesis

EB1002

EB1002 Chemical Structure

CAS No. : 2770688-48-5

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EB1002 Related Antibodies

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Description

EB1002 is a highly selective, long-acting NK2R agonist. EB1002 exerts central appetite suppression, increases peripheral energy expenditure and enhances insulin sensitivity, which effectively reduces body weight, improves glucose and lipid metabolism, with favorable safety profiles. EB1002 can be used for research on diseases such as obesity and type 2 diabetes[1][2].

IC50 & Target[1]

NK2R

 

In Vitro

EB1002 (120 minutes) selectively activates human/mouse NK2R in COS-7 cells, without inducing signal transduction in human/mouse NK1R or NK3R receptors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EB1002 Related Antibodies
In Vivo

EB1002 (40-325 nmol/kg s.c.; 2 nmol ICV; single dose, daily, every other day; 7/12/21 days) induces weight loss via increased energy expenditure and appetite suppression, improves insulin sensitivity, and has effects independent of leptin, Mc4r, and Ucp1[1].
EB1002 (s.c. single dose; s.c. daily, 7 days; i.c.v. single dose) reduces body weight via decreased food intake and increased energy expenditure, improves glucose metabolism, and preserves lean mass in obese Mus musculus[2]. Wait, no, the original text uses "obese mice" not the Latin name. Correcting to follow the rules strictly without altering content:\n\nEB1002 (s.c. single dose; s.c. daily, 7 days; i.c.v. single dose) reduces body weight via decreased food intake and increased energy expenditure, improves glucose metabolism, and preserves lean mass in obese mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57Bl/6 NRj (male and female, ≥8 weeks old); B6.V-Lepob/JRj (ob/ob, male and female, ≥8 weeks old); B6.129S4-Mc4rtm1Lowl/J (Mc4r-knockout, ≥8 weeks old); B6N-Tacr2tm1Zpg (Nk2r-floxed, ≥8 weeks old); Ucp1-knockout (≥8 weeks old, thermoneutral housing); CD-1 mice (≥8 weeks old); diet-induced obesity model (60% HFD for ≥12 weeks)[1]
Dosage: 40 nmol/kg; 325 nmol/kg; 2 nmol (ICV)
Administration: s.c. (single dose); s.c. (daily, 7/12/21 days); s.c. (every other day, 21 days); ICV (single dose, 1.5 μl artificial CSF)
Result: Increased oxygen consumption, fatty acid oxidation, and core temperature; reduced food intake, respiratory exchange ratio (RER), and body weight in DIO mice (single 325 nmol/kg s.c.).
Reduced body weight (solely fat mass loss, lean mass spared), improved glucose tolerance, and increased insulin sensitivity (50% higher glucose infusion rate during clamp) in DIO mice (daily 325 nmol/kg s.c. for 7 days).
Reduced food intake, body weight, and blood glucose in ob/ob mice (325 nmol/kg s.c.).
Reduced food intake and body weight but not oxygen consumption or blood glucose in ob/ob mice (ICV 2 nmol).
Abolished effects in Nk2r-knockout mice. Reduced body weight, blood glucose, RER, and increased fatty acid oxidation, oxygen consumption, and body temperature in Ucp1-knockout mice at thermoneutrality (single 325 nmol/kg s.c.) compared to vehicle and pair-fed controls.
Molecular Weight

1537.83

Formula

C73H124N12O23
CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (32.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 48.78 mg/mL (31.72 mM; adjust pH to 3 with 1 M HCl)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6503 mL 3.2513 mL 6.5027 mL
5 mM 0.1301 mL 0.6503 mL 1.3005 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.98%

SDS (252 KB)

COA (252 KB) RP-HPLC (253 KB) MS (71 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.6503 mL 3.2513 mL 6.5027 mL 16.2567 mL
5 mM 0.1301 mL 0.6503 mL 1.3005 mL 3.2513 mL
10 mM 0.0650 mL 0.3251 mL 0.6503 mL 1.6257 mL
15 mM 0.0434 mL 0.2168 mL 0.4335 mL 1.0838 mL
20 mM 0.0325 mL 0.1626 mL 0.3251 mL 0.8128 mL
25 mM 0.0260 mL 0.1301 mL 0.2601 mL 0.6503 mL
30 mM 0.0217 mL 0.1084 mL 0.2168 mL 0.5419 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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EB1002 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EB10022770688-48-5EB 1002EB-1002Neurokinin ReceptorNK receptorTachykinin receptorNK2leptinobeseob/ob micediet-induced obese miceInhibitorinhibitorinhibit

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