HDAC-MB
Based on 1 Customer Validation
HDAC-MB a probe that is activated by HDAC6 and can detect and eliminate glioma cells through activation by HDAC6. HDAC-MB reveals antimetastatic and antiproliferative properties, inhibits glioma invasion and induces cellular apoptosis.
For research use only. We do not sell to patients.
- Purity: 99.42%
- Formula: C32H38N4O5S
- Molecular Weight:590.73
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Storage:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
HDAC-MB (10 μM) can be activated by HDAC6, produces near-infrared fluorescence and images HDAC6[1].
HDAC-MB (0-5 μM) is phototoxic and activated by HDAC6 in HeLab and U251 cells[1].
HDAC-MB (5 μM) inhibits the glioma cells migration, invasion and proliferation through the synergestic effect of MAO A inhibition and PDT, induces thereby glioma cellsapoptosis[1].Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
Typical usage scenarios include :
Target cell lines: U251 glioma cells and HeLa cells.
Concentration: 1 - 10 μM (typically 5 μM is used).
Incubation time: Approximately 24 hours (for example, U251 cells, 24 hours).
Methodology:
Imaging: This probe emits near-infrared fluorescence (when excited at a concentration of 10 μM) after being activated by HDAC6.
Photodynamic therapy (PDT): HDAC-MB exhibits enhanced anti-migration and anti-proliferation effects under light exposure, reducing cell migration by up to 70%.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa
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Concentration:0-5 μM
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Incubation Time:24 h
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Result:Revealed a dose-dependent cytotoxicity.
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Cell Line:U251 cells
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Concentration:1 μM
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Incubation Time:24 h
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Result:Reduced the cell migration by 40% with no irradiation, reduced the cell migration by 70% with light irradiation.
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Cell Line:U251 cells
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Concentration:15 μM
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Incubation Time:24 h
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Result:Induced apoptosis upon NIR light irradiation.
Chemical Information
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Appearance Solid
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Molecular Weight 590.73
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Formula C32H38N4O5S
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Color Light blue to blue
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SMILES
CC(NCCCCCC(OC1=CC=C(C=C1)COC(N2C3=CC=C(C=C3SC4=CC(N(C)C)=CC=C42)N(C)C)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 100 mg/mL (169.28 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6928 mL | 8.4641 mL | 16.9282 mL | 42.3205 mL |
| 5 mM | 0.3386 mL | 1.6928 mL | 3.3856 mL | 8.4641 mL | |
| 10 mM | 0.1693 mL | 0.8464 mL | 1.6928 mL | 4.2321 mL | |
| 15 mM | 0.1129 mL | 0.5643 mL | 1.1285 mL | 2.8214 mL | |
| 20 mM | 0.0846 mL | 0.4232 mL | 0.8464 mL | 2.1160 mL | |
| 25 mM | 0.0677 mL | 0.3386 mL | 0.6771 mL | 1.6928 mL | |
| 30 mM | 0.0564 mL | 0.2821 mL | 0.5643 mL | 1.4107 mL | |
| 40 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0580 mL | |
| 50 mM | 0.0339 mL | 0.1693 mL | 0.3386 mL | 0.8464 mL | |
| 60 mM | 0.0282 mL | 0.1411 mL | 0.2821 mL | 0.7053 mL | |
| 80 mM | 0.0212 mL | 0.1058 mL | 0.2116 mL | 0.5290 mL | |
| 100 mM | 0.0169 mL | 0.0846 mL | 0.1693 mL | 0.4232 mL |
HDAC-MB Related Classifications
HY-163290 Related Classifications
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