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Cat. No.: HY-172195

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FS-694

FS-694, a chemical probe, is the inhibitor for p38α with an IC₅₀ of 0.2 nM. FS-694 inhibits the TNFα release in human whole blood with an IC₅₀ of 35.0 nM.

For research use only. We do not sell to patients.

CAS No.: 1417404-27-3
Formula: C₃₅H₃₃FN₄O₄
Molecular Weight:592.66

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (266 KB)

FS-694 Chemical Structure

CAS No.: 1417404-27-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

FS-694, a chemical probe, is the inhibitor for p38α with an IC₅₀ of 0.2 nM. FS-694 inhibits the TNFα release in human whole blood with an IC₅₀ of 35.0 nM^[1].

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	4.3 nM Compound: 2	Inhibition of NanoBRET kinase tracer K4 binding to full-length human C-terminal NanoLuc-tagged p38alpha MAPK expressed in HEK293T cells incubated for 2 hrs by NanoBRET NanoGlo substrate based NanoBRET assay Inhibition of NanoBRET kinase tracer K4 binding to full-length human C-terminal NanoLuc-tagged p38alpha MAPK expressed in HEK293T cells incubated for 2 hrs by NanoBRET NanoGlo substrate based NanoBRET assay	[PMID: 32462866]
Sf9	IC₅₀	4 nM Compound: 2	Inhibition of recombinant human full-length N-terminal GST-tagged p38alpha MAPK expressed in baculovirus infected Sf9 insect cells using p38 peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by ADP-glo Inhibition of recombinant human full-length N-terminal GST-tagged p38alpha MAPK expressed in baculovirus infected Sf9 insect cells using p38 peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by ADP-glo	[PMID: 32462866]

Chemical Information

CAS No. 1417404-27-3
Molecular Weight 592.66
Formula C₃₅H₃₃FN₄O₄
SMILES

FC(C=C1)=C(NC(C2=CC=CC=C2)=O)C=C1NC3=CC(CCC(C=CC(C(NCCN4CCOCC4)=O)=C5)=C5C6=O)=C6C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Walter NM, et al., Design, Synthesis, and Biological Evaluation of Novel Type I1/2 p38α MAP Kinase Inhibitors with Excellent Selectivity, High Potency, and Prolonged Target Residence Time by Interfering with the R-Spine. J Med Chem. 2017 Oct 12;60(19):8027-8054. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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