G3-YSD 

G3-YSD is a cGAS agonist. G3-YSD directly interacts with cGAS to enhance its enzymatic activity, promote the conversion of ATP and GTP into cGAMP, and trigger STING-dependent IFN-α/β secretion. G3-YSD acts as a viral mimic to replace actual viral DNA. G3-YSD is applicable to research related to long COVID and type 1 human immunodeficiency virus infection^[1][2].

Stimulation with G3-YSD (1 mg/mL; 24 h) induces significant type I interferon (IFN-I) biological activity in peripheral blood mononuclear cells (PBMCs) from Microx long COVID patients^[1].
G3-YSD (0.8 µg/mL; 20 h) potently induces IFN-α secretion from Chloroquine (HY-17589A)-treated human peripheral blood mononuclear cells in a guanosine-dependent manner. It maintains stable activity across a core double-strand length range of 12-28 bp and tolerates small mismatches/bulges in the core double strand^[2].
G3-YSD (0.8 µg/mL; 20-36 h) potently induces IFN-α secretion in primary human monocytes and monocyte-derived macrophages, and its activity in macrophages is comparable to that of long-chain HIV-1 ssDNA^[2].
G3-YSD (1.3 µg/mL; 20 h) induces IFN-α/β secretion in the human monocyte cell line THP-1 solely via the cGAS-STING signaling axis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8088.29

2780391-08-2

Solid

White to off-white

[DNA, d(G-G-G-T-A-T-A-T-A-T-A-T-G-C-A-T-A-T-A-T-A-T-A-G-G-G)]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Humer B, et al. Exaggerated IFN-I Response in Long COVID PBMCs Following Exposure to Viral Mimics. J Clin Immunol. 2025;46(1):5. Published 2025 Dec 10.

  [2]. Herzner AM, et al. Sequence-specific activation of the DNA sensor cGAS by Y-form DNA structures as found in primary HIV-1 cDNA. Nat Immunol. 2015;16(10):1025-1033.  [Content Brief]