2-Bromoacetamide
Based on 1 Customer Validation
2-Bromoacetamide is a disinfection byproduct. 2-Bromoacetamide can inactivate liver alcohol dehydrogenase and interfere with microtubule and actin cytoskeletal function. 2-Bromoacetamide is a potent developmental toxicant in animals.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.77%
- No. CAS: 683-57-8
- Fòrmula: C2H4BrNO
- Peso molecular:137.96
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
In Vitro
2-Bromoacetamide inactivates liver alcohol dehydrogenase[1].
2-Bromoacetamide (250 μM-1 mM) reduces the egg cylinder formation rate in a dose-dependent manner, disrupts cell lineage differentiation in mouse embryo[2].
2-Bromoacetamide (3.5-4 mg/L; 8-24 h) reduces the first polar body extrusion rate in a dose-dependent manner, disrupts spindle structure, interferes with microtubule and actin cytoskeleton functions in mouse and human oocyte[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 683-57-8
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Appearance Solid
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Peso molecular 137.96
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Fòrmula C2H4BrNO
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Color White to off-white
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SMILES
O=C(N)CBr
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (724.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.12 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (641 KB)
- English - EN (641 KB)
- Français - FR (641 KB)
- Deutsch - DE (641 KB)
- Norwegian - NO (641 KB)
- Español - ES (641 KB)
- Swedish - SV (641 KB)
- Italian - IT (641 KB)
- Korean - KR (641 KB)
- Portuguese - PT (641 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Fries RW, et al. Activation of liver alcohol dehydrogenases by imidoesters generated in solution. Biochemistry. 1975;14(23):5233‐5238. [Content Brief]
[2]. Zhang C, et al. Developmental toxicity of 2-bromoacetamide on peri- and early post-implantation mouse embryos in vitro. Ecotoxicol Environ Saf. 2023 Mar 1;252:114612. [Content Brief]
[3]. Zhang C, et al. 2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton. Ecotoxicol Environ Saf. 2024 Mar 1;272:116105. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.2485 mL | 36.2424 mL | 72.4848 mL | 181.2119 mL |
| 5 mM | 1.4497 mL | 7.2485 mL | 14.4970 mL | 36.2424 mL | |
| 10 mM | 0.7248 mL | 3.6242 mL | 7.2485 mL | 18.1212 mL | |
| 15 mM | 0.4832 mL | 2.4162 mL | 4.8323 mL | 12.0808 mL | |
| 20 mM | 0.3624 mL | 1.8121 mL | 3.6242 mL | 9.0606 mL | |
| 25 mM | 0.2899 mL | 1.4497 mL | 2.8994 mL | 7.2485 mL | |
| 30 mM | 0.2416 mL | 1.2081 mL | 2.4162 mL | 6.0404 mL | |
| 40 mM | 0.1812 mL | 0.9061 mL | 1.8121 mL | 4.5303 mL | |
| 50 mM | 0.1450 mL | 0.7248 mL | 1.4497 mL | 3.6242 mL | |
| 60 mM | 0.1208 mL | 0.6040 mL | 1.2081 mL | 3.0202 mL | |
| 80 mM | 0.0906 mL | 0.4530 mL | 0.9061 mL | 2.2651 mL | |
| 100 mM | 0.0725 mL | 0.3624 mL | 0.7248 mL | 1.8121 mL |
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