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FITC

(Synonyms: Fluorescein 5-isothiocyanate)
92 Cited Publications
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Based on 92 publication(s) in Google Scholar

FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model.

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  • Pureza: 98.60%
  • No. CAS: 3326-32-7
  • Fòrmula: C21H11NO5S
  • Peso molecular:389.38
  • Almacenamiento:

    -20°C, protect from light, stored under nitrogen

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

  • FITC Chemical Structure
    FITC Chemical Structure
  • Experimental Validation Results for FITC
  • Experimental Validation Results for FITC
  • Experimental Validation Results for FITC
  • Experimental Validation Results for FITC
  • Experimental Validation Results for FITC
  • Experimental Validation Results for FITC
No. CAS: 3326-32-7
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  • FITC Chemical Structure
    FITC Chemical Structure
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    FITC purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 May 5:322:123391. [Abstract]

    Customer Validation
     Design and construction of RTBs for remodeling CSC plasticity. Fluorescence emission spectra of FITC-RTB-RhB at 488 nm. (FITC: HY-66019; RhB, Rhodamine B, HY-Y0016)

  • 1/16

    FITC purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Mar;9(8):e2104344. [Abstract]

    Customer Validation
     For detection of localization of WBC100 in cells, FITC labeled WBC100 (WBC100‐FITC) is used. HEK293T cells are transfected with the indicated plasmids for 48 h. Cells are treated with 1 μM WBC100‐FITC or FITC for 24 h.

  • 1/16

    FITC purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2022 Feb 2;20(1):61. [Abstract]

    Customer Validation
     To exam the ability of anti-PD-L1 antibodies (aPD-L1) nanovesicles to binding to tumor cells, B16F10 cells are cultured for 24 h. FITC-labeled aPD-L1 nanovesicles or nanovesicles are incubated with the cancer cells for 3 h.

  • 1/16

    FITC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Nov 5;12(1):6426. [Abstract]

    Customer Validation
     Cinnamate acetate (CIA) was labeled with FITC. Distribution of CIA/FITC in root cells of wild-type, ala7 mutant and complemented Arabidopsis seedlings. The seedlings were treated with CIA/FITC (5 μg/mL) and FM4-64 (8 μM) for 1 h, 2 h, and 8 h.

  • 1/16

    FITC purchased from MedChemExpress. Usage Cited in: ACS Nano. 2020 Nov 24;14(11):14907-14918. [Abstract]

    Customer Validation
     FITC-labeled MnO2/BPD are added at a concentration of 50 μg/mL and incubated for another 4 h to allow the nanoparticles to enter the cells. By conjugating FITC to MnO2/BPD, FL could be monitored at 488 nm, and the vessels could be observed at 660 nm by Evans Blue staining.

  • 1/16

    FITC purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2020, 127870.

    Customer Validation
     FITC (1 mg/mL) solution was prepared with dimethyl sulfoxide. Then FITC solution was added dropwise into laccase (5 mg/mL) solution, and the mixed solution was stirred at 4 °C for 4 h. Laccase was labelled by FITC and exhibited green emissions.

  • FITC