IOWH-032
Based on 4 publication(s) in Google Scholar
IOWH-032 is a a synthetic anti-secretory molecule, is a potent CFTR inhibitor with an IC50 value of 8 μM. IOWH-032 also is a anti-diarrheal agent.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.63%
- No. CAS: 1191252-49-9
- Fòrmula: C22H15Br2N3O4
- Peso molecular:545.18
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) IOWH-032
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Actividad biológica
Descripciòn
In Vitro
IOWH-032 (10 μM; 0-72 h) increases the ACE-2 expression in SARS-CoV-2 infected CFBE41o- WT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CFBE41o- cells
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Concentration:10 µM
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Incubation Time:0, 24, 48, 72 h
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Result:Increased the ACE-2 expression in SARS-CoV-2 infected CFBE41o- WT cells in a time-dependent manner.
Ensayo clínico
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 1191252-49-9
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Appearance Solid
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Peso molecular 545.18
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Fòrmula C22H15Br2N3O4
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Color White to off-white
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SMILES
BrC1=C(O)C(Br)=CC(C2=NOC(C(NCC3=CC=C(OC4=CC=CC=C4)C=C3)=O)=N2)=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
Solvente y solubilidad
In Vitro:
DMSO : ≥ 100 mg/mL (183.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[2]. de Hostos EL, et al. Developing novel antisecretory drugs to treat infectious diarrhea. Future Med Chem. 2011 Aug;3(10):1317-25. [Content Brief]
[3]. Lotti V, et al. CFTR Modulation Reduces SARS-CoV-2 Infection in Human Bronchial Epithelial Cells. Cells. 2022 Apr 15;11(8):1347. [Content Brief]
[4]. Thiagarajah JR, et al. CFTR inhibitors for treating diarrheal disease. Clin Pharmacol Ther. 2012 Sep;92(3):287-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8343 mL | 9.1713 mL | 18.3426 mL | 45.8564 mL |
| 5 mM | 0.3669 mL | 1.8343 mL | 3.6685 mL | 9.1713 mL | |
| 10 mM | 0.1834 mL | 0.9171 mL | 1.8343 mL | 4.5856 mL | |
| 15 mM | 0.1223 mL | 0.6114 mL | 1.2228 mL | 3.0571 mL | |
| 20 mM | 0.0917 mL | 0.4586 mL | 0.9171 mL | 2.2928 mL | |
| 25 mM | 0.0734 mL | 0.3669 mL | 0.7337 mL | 1.8343 mL | |
| 30 mM | 0.0611 mL | 0.3057 mL | 0.6114 mL | 1.5285 mL | |
| 40 mM | 0.0459 mL | 0.2293 mL | 0.4586 mL | 1.1464 mL | |
| 50 mM | 0.0367 mL | 0.1834 mL | 0.3669 mL | 0.9171 mL | |
| 60 mM | 0.0306 mL | 0.1529 mL | 0.3057 mL | 0.7643 mL | |
| 80 mM | 0.0229 mL | 0.1146 mL | 0.2293 mL | 0.5732 mL | |
| 100 mM | 0.0183 mL | 0.0917 mL | 0.1834 mL | 0.4586 mL |
IOWH-032 Related Classifications
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