NS309
Based on 1 publication(s) in Google Scholar
NS309 is a potent and selective activator of the Ca2+-activated SK/IK potassium channels, but displays no activity at BK channels.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.09%
- No. CAS: 18711-16-5
- Fòrmula: C8H4Cl2N2O2
- Peso molecular:231.04
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Publications Citing Use of MedChemExpress (MCE) NS309
More
Actividad biológica
EC50: 0.62 μM (SK2)[1], 0.3 μM (SK3)[2], ～20 nM (KCa3.1/SK4)[3], ～600 nM (KCa2/SK channels)[3], 10 nM (hIK)[4]
NS309 (40 nM) activates both hSK3 and hIK channels, with the largest effect on hIK channels (8.5-fold increase in current compares to 1.9-fold increase in current for hSK3 channels)[2].
NS309 (10 μM) significantly increases the whole cell SK currents and hyperpolarized detrusor smooth muscle (DSM) cells resting membrane potential[5].
NS309 inhibits the spontaneous phasic contraction amplitude, force, frequency, duration and tone of isolated DSM strips in a concentration-dependent manner[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult New Zealand white rabbits (2.5-3.0 kg)[6]
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Dosage:2 mg/kg
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Administration:Intraperitoneal injection
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Result:Significantly improved neurological outcome of SCI/R challenged rabbits.
Chemical Information
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No. CAS 18711-16-5
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Appearance Solid
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Peso molecular 231.04
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Fòrmula C8H4Cl2N2O2
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Color Light yellow to yellow
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SMILES
O=C1NC2=C(C=CC(Cl)=C2Cl)/C1=N/O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Biochem Pharmacol
Dysfunction in the SOCE/EDH-mediated vasorelaxation of mesenteric arteries impairs intestinal barrier integrity in irritable bowel syndrome. [Abstract]2026 Jun 18;251(Pt 2):118179. PMID: 42315077
Solvente y solubilidad
DMSO : 100 mg/mL (432.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Specific enhancement of SK channel activity selectively potentiates the afterhyperpolarizing current I(AHP) and modulates the firing properties of hippocampal pyramidal neurons. [Content Brief]
[2]. C Hougaard, et al. Selective positive modulation of the SK3 and SK2 subtypes of small conductance Ca2+-activated K+ channels. Br J Pharmacol. 2007 Jul; 151(5): 655–665. [Content Brief]
[3]. Nichole Coleman, et al. New Positive Ca2+-Activated K+ Channel Gating Modulators with Selectivity for KCa3.1. Mol Pharmacol. 2014 Sep; 86(3): 342–357. [Content Brief]
[4]. Dorte Strøbaek, et al. Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim Biophys Acta. 2004 Oct 11;1665(1-2):1-5. [Content Brief]
[5]. Shankar P Parajuli, et al. NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels. Br J Pharmacol. 2013 Apr; 168(7): 1611–1625. [Content Brief]
[6]. Jie Zhu, et al. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019; 10: 325. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3283 mL | 21.6413 mL | 43.2825 mL | 108.2064 mL |
| 5 mM | 0.8657 mL | 4.3283 mL | 8.6565 mL | 21.6413 mL | |
| 10 mM | 0.4328 mL | 2.1641 mL | 4.3283 mL | 10.8206 mL | |
| 15 mM | 0.2886 mL | 1.4428 mL | 2.8855 mL | 7.2138 mL | |
| 20 mM | 0.2164 mL | 1.0821 mL | 2.1641 mL | 5.4103 mL | |
| 25 mM | 0.1731 mL | 0.8657 mL | 1.7313 mL | 4.3283 mL | |
| 30 mM | 0.1443 mL | 0.7214 mL | 1.4428 mL | 3.6069 mL | |
| 40 mM | 0.1082 mL | 0.5410 mL | 1.0821 mL | 2.7052 mL | |
| 50 mM | 0.0866 mL | 0.4328 mL | 0.8657 mL | 2.1641 mL | |
| 60 mM | 0.0721 mL | 0.3607 mL | 0.7214 mL | 1.8034 mL | |
| 80 mM | 0.0541 mL | 0.2705 mL | 0.5410 mL | 1.3526 mL | |
| 100 mM | 0.0433 mL | 0.2164 mL | 0.4328 mL | 1.0821 mL |
NS309 Related Classifications
HY-15416 Related Classifications
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