NSC319726
Based on 4 publication(s) in Google Scholar
NSC319726 (ZMC1) is a mutant p53R175 reactivator; inhibits growth of fibroblasts expressing the p53R175 mutation (IC50 = 8 nM); shows no inhibition for p53 wild-type cells.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.60%
- No. CAS: 71555-25-4
- Fòrmula: C11H14N4S
- Peso molecular:234.32
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) NSC319726
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Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
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IF
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In Vivo Efficacy Study
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WB
Actividad biológica
IC50: 8 nM (mutant p53R175 reactivator)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | CC50 |
<0.02 μM
Compound: NSC319726
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Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
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10.21203/rs.3.rs-23951/v1 |
| Caco-2 | IC50 |
<0.02 μM
Compound: NSC319726
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Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
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10.21203/rs.3.rs-23951/v1 |
| TOV112D | EC50 |
0.005 μM
Compound: ZMC1
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Growth inhibition of human TOV112D cells harbouring p53 R175H mutant incubated for 3 days by MTS assay
Growth inhibition of human TOV112D cells harbouring p53 R175H mutant incubated for 3 days by MTS assay
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[PMID: 33538587] |
For NSC319726, the effect was even greater as the IC50 for the 175 mutant was 8 nM while the IC50 of the WT was not reached. NSC319726 did not induce WT p53 protein levels or transcriptional activity as common cytotoxic agents such as etoposide do in vitro. NSC319726 exhibited a much higher sensitivity for the MEF-p53R172H/R172H cell line as compared to the p53+/+ and p53-/- controls. NSC319726 treatment of a MEF cell line derived from p53R172H/R172H mice resulted in a? loss of PAB240 immunoflouresence staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 71555-25-4
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Appearance Solid
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Peso molecular 234.32
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Fòrmula C11H14N4S
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Color Off-white to light yellow
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SMILES
C/C(C1=NC=CC=C1)=N\NC(N2CCC2)=S
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Synonyms
ZMC1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Cancer Cell
2025 May 12;43(5):937-954.e9. PMID: 40054467 -
J Adv Res
Targeting myofibroblast copper vulnerability reverses pulmonary fibrosis via METTL3-directed STAT6 m6A driving cuproptosis. [Abstract]2026 May 21:S2090-1232(26)00436-4. PMID: 42173361 -
J Exp Bot
Transcriptomic and functional analyses reveal a complex unexplored landscape of Botrytis cinerea colonization in rose. [Abstract]2025 May 17:eraf219. PMID: 40380888 -
Cell Biol Toxicol
Retinoic acid mitigates the NSC319726-induced spermatogenesis dysfunction through cuproptosis-independent mechanisms. [Abstract]2024 May 1;40(1):26. PMID: 38691186
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 5 weeks) showed no significant variation in the body weight of mice. the testis index and pregnancy rate decreased significantly, and there was a decrease in sperm counts with an increased sperm abnormality rate in Male ICR mice
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 5 weeks) demonstrated a reduction in the quantity of sperm in the caput and cauda epididymis in Male ICR mice. Hematoxylin–eosin staining of histopathological changes in the epididymis.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 5 weeks) decrease the expression of DDX4 and SYCP3 in testis in ICR mice.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 5 weeks) exhibited significantly lower testosterone levels in serum and testis tissues than in the control group in ICR mice.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 5 weeks) decreased the protein expression of CYP17A1, ZO-1, Vinculin, and β-catenin in testes.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 10 weeks) and in the final five weeks, the mice were administered intraperitoneal injections of either 5 mg/kg RA or 0.03 mg/mL of TTM in their drinking water. NSC319726 increased the protein expression of PDX1 in testis.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 (1 mg/kg; IP; daily for 10 weeks) and in the final five weeks, the mice were administered intraperitoneal injections of either 5 mg/kg RA or 0.03 mg/mL of TTM in their drinking water. NSC319726 reduced germs and total germ cells in seminiferous tubule.
NSC319726 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2024 May 1;40(1):26. [Abstract]
NSC319726 decreased the protein expression of STRA8 and RDH10 in testis. NSC319726 (1 mg/kg; IP; daily for 10 weeks) and in the final five weeks, the mice were administered intraperitoneal injections of either 5 mg/kg RA or 0.03 mg/mL of TTM in their drinking water. NSC319726 reduced germs and total germ cells in seminiferous tubule.
Solvente y solubilidad
DMSO : 31.25 mg/mL (133.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.2677 mL | 21.3383 mL | 42.6767 mL | 106.6917 mL |
| 5 mM | 0.8535 mL | 4.2677 mL | 8.5353 mL | 21.3383 mL | |
| 10 mM | 0.4268 mL | 2.1338 mL | 4.2677 mL | 10.6692 mL | |
| 15 mM | 0.2845 mL | 1.4226 mL | 2.8451 mL | 7.1128 mL | |
| 20 mM | 0.2134 mL | 1.0669 mL | 2.1338 mL | 5.3346 mL | |
| 25 mM | 0.1707 mL | 0.8535 mL | 1.7071 mL | 4.2677 mL | |
| 30 mM | 0.1423 mL | 0.7113 mL | 1.4226 mL | 3.5564 mL | |
| 40 mM | 0.1067 mL | 0.5335 mL | 1.0669 mL | 2.6673 mL | |
| 50 mM | 0.0854 mL | 0.4268 mL | 0.8535 mL | 2.1338 mL | |
| 60 mM | 0.0711 mL | 0.3556 mL | 0.7113 mL | 1.7782 mL | |
| 80 mM | 0.0533 mL | 0.2667 mL | 0.5335 mL | 1.3336 mL | |
| 100 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0669 mL |
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