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Rhod-2 AM

42 Cited Publications
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Based on 42 publication(s) in Google Scholar

Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm.

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  • No. CAS: 145037-81-6
  • Fòrmula: C52H59BrN4O19
  • Peso molecular:1123.94
  • Almacenamiento:

    -20°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

  • Rhod-2 AM Chemical Structure
    Rhod-2 AM Chemical Structure
  • Experimental Validation Results for Rhod-2 AM
  • Experimental Validation Results for Rhod-2 AM
  • Experimental Validation Results for Rhod-2 AM
  • Experimental Validation Results for Rhod-2 AM
  • Experimental Validation Results for Rhod-2 AM
No. CAS: 145037-81-6
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Encuentre su distribuidor local Your local distributor: Hycultec GmbHHycultec
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IFCell Imaging/Staining
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  • Rhod-2 AM Chemical Structure
    Rhod-2 AM Chemical Structure
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  • 1/16

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030. [Abstract]

    Customer Validation
     CLSM images showing membrane integrity and calcium levels of mitochondria in 4T1 cells treated with various nanoreactors. Rhod‐2 AM was dissolved in DMSO containing 0.02% Pluronic F127 and diluted using PBS to a final concentration of 5 µM. 4T1 cells were seeded in confocal dishes and cultured for 24 h. Nanoreactors (200 µg/mL) were added to the culture for an additional 24 h. Following three washes with PBS, the cells were incubated with the probe for 30 min, followed by imaging with CLSM.

  • 1/16

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]

    Customer Validation
     To detect the level of Ca2+ in mitochondria, C. albicans cells were stained with Rhod-2 AM (5 μM, 30 min) at 37 °C. The images were acquired utilizing a confocal microscope. The excitation and emission wavelengths for the fluorescent probes used in this study are as follows: ER-Tracker Red (587 nm/615 nm), Rhod-2AM (549 nm/578 nm).

  • 1/16

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 21;11(47):eadz4126. [Abstract]

    Customer Validation
     RAW 264.7 cells were harvested and stained with a mitochondrial Ca2+ indicator (Rhod-2 AM) at room temperature for 30 min.

  • 1/16

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 May 7:S2090-1232(24)00175-9. [Abstract]

    Customer Validation
     To detect intracellular Ca2+, cells were treated with 1 μM of Rhod-2 AM fluorescent probes and subsequently washed. The intracellular Ca2+ levels were quantified.

  • 1/16

    Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948. [Abstract]

    Customer Validation
     Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa2O3 treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.

  • Rhod-2 AM