Acapatamab
Based on 1 Customer Validation
Acapatamab (AMG-160) is an anti-CD3E/FOLH1 monoclonal antibody.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.35%
- No. CAS: 2314491-93-3
- Peso molecular:105.72 kDa
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
Actividad biológica
Descripciòn
Isotype
(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
CD3E & FOLH1
In Vitro
Acapatamab (AMG-160; 0-10000 pM; 48 h) induces T-cell activation and increases in CD25, CD69, 4-1BB, and PD-1 expression on T cells[1].
Acapatamab (0-10000 pM; 48 h; T-cell) induces dose-dependent secretion of cytokines, including IL2, IL4, IL6, IL10, IFNγ, and TNFα[1].
Acapatamab (0-10000 pM; 48 h) induces potent lysis of prostate-specific membrane antigen (PSMA)-positive prostate cancer cell lines in cocultures with PBMC, with EC50 values of 6-42 pM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD/SCID mice with 22Rv-1 CRPC xenografts[1]
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Dosage:0.02, 0.2, and 2 mg/kg
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Administration:Intravenous injection (days 16, 23, and 31)
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Result:Had antitumor activity against established CRPC tumors.
Ensayo clínico
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Aplicación
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1×106 Jurkat cells labelling CD3E (red) with Acapatamab (HY-P99418). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control.
Chemical Information
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No. CAS 2314491-93-3
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Appearance Liquid
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Peso molecular 105.72 kDa
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Color Colorless to light yellow
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SMILES
[Acapatamab]
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Synonyms
AMG-160
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Envío
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Referencias
[1]. Acapatamab
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)