Referencia número: HY-134923

CA77.1

8 Cited Publications

Revisión del cliente

Based on 8 publication(s) in Google Scholar

CA77.1 is a potent, brain-penetrant and orally active chaperone-mediated autophagy (CMA) activator with favorable pharmacokinetics. CA77.1 is a derivative of AR7 (HY-101106) and can increase the expression of the lysosomal receptor LAMP2A in?lysosomes. CA77.1 improves behavior and neuropathology in PS19 mice model and can be used for alzheimer's?disease research.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza: 99.92%
No. CAS: 2412270-22-3
Fòrmula: C₁₆H₁₂ClN₃O
Peso molecular:297.74

Almacenamiento:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

CA77.1 Chemical Structure

No. CAS: 2412270-22-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

In Vitro

CA77.1 (0-30 μM; 16 hours) activates CMA in a dose-and time-dependent manner to NIH 3T3 cells stably expressing the KFERQ-PS-Dendra reporter. The CMA activity is quantified as the average of fluorescent puncta per cell^[1].
CA77.1 (20 μM; 6 hours) does not alter on LC3-II expression, and does not effects autophagic flux in NIH 3T3 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CA77.1 (oral gavage; 10 mg/kg;single dose) demonstrates brain penetrance with favorable pharmacokinetics. The C_max, AUC_last, T_max and T_1/2 are 3534 ng/g, 8338 h*ng/g, 1 hour and 1.89 hour, respectively^[1].
CA77.1 (oral gavage; 30 mg/kg; 6 months) normalizes the previously described locomotor hyperactivity of PS19 mice to control levels. And it reduces the levels and number of neurons containing pathogenic tau conformations in the hippocampus, amygdala, and piriform cortex. And the higher number of microglial cells and presence of large Iba1-positive cells with rod-like dystrophic morphology in vehicle-treated PS19 mice are reduced upon CA77.1 treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

9-month-old CTR or PS19 mice^[1]
Dosage:

30 mg/kg
Administration:

Oral gavage; 30 mg/kg; 6 months
Result:

Improved behavior and neuropathology in a mouse model of frontotemporal-dementia-related proteotoxicity.

Chemical Information

No. CAS 2412270-22-3
Appearance Solid
Peso molecular 297.74
Fòrmula C₁₆H₁₂ClN₃O
Color Off-white to light yellow
SMILES

CC(NC1=CC=C(C2=NC3=CC=C(Cl)C=C3N=C2)C=C1)=O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (8)

All WBIn Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

Autophagy

Activation of chaperone-mediated autophagy suppresses glioblastoma by promoting wild-type IDH1/isocitrate dehydrogenase 1 degradation. [Abstract]

2025 Nov 30:1-21. PMID: 41231102
Sci Transl Med

Chaperone-mediated autophagy supports organ regeneration and fibroblast quiescence in mouse models of fibrosis. [Abstract]

2026 Feb 18;18(837):eads9597. PMID: 41706873

CA77.1 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2026 Feb 18;18(837):eads9597. [Abstract]

CA77.1 (20 μM; 24-48 h) decreased the expression of FN and α-SMA, and increased LAMP2A in TGF-β-treated NIH3T3 cells.

CA77.1 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2026 Feb 18;18(837):eads9597. [Abstract]

CA77.1 (20 μM; 24-48 h) decreased the expression of FN and α-SMA, and increased LAMP2A in TGF-β-treated LX-2 cells.

CA77.1 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2026 Feb 18;18(837):eads9597. [Abstract]

CA77.1 (10 mg/kg; i.p.; once daily for 2 weeks) improved respiratory function, as reflected by increased tidal volume and minute ventilation in pulmonary fibrosis mice.

CA77.1 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2026 Feb 18;18(837):eads9597. [Abstract]

CA77.1 (10 mg/kg; i.p.; once daily for 2 weeks) reduced fibrotic pathology in pulmonary fibrosis mice.

CA77.1 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2026 Feb 18;18(837):eads9597. [Abstract]

CA77.1 (10 mg/kg; i.p.; once daily for 2 weeks) reduced FN, LAMP2A, and α-SMA expression in lung tissue of pulmonary fibrosis mice.
Sci Adv

Deficient chaperone-mediated autophagy facilitates LPS-induced microglial activation via regulation of the p300/NF-κB/NLRP3 pathway. [Abstract]

2023 Oct 6;9(40):eadi8343. PMID: 37801503
Cell Rep

LAMP2A-mediated neuronal hyperexcitability by enhancing NKAβ1 degradation underlies depression-induced allodynia. [Abstract]

2025 Apr 2;44(4):115489. PMID: 40178973
Cell Rep

Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal. [Abstract]

2023 Aug 16;42(8):112998. PMID: 37590146
Am J Physiol Cell Physiol

Central activation of chaperone-mediated autophagy reduces appetite by fine-tuning hypothalamic amino acid pools: new insights from fish. [Abstract]

2026 Mar 1;330(3):C590-C604. PMID: 41525106
J Virol

Heat shock protein A1 inhibits the replication of foot-and-mouth disease virus by degrading viral RNA polymerase 3D through chaperone-mediated autophagy. [Abstract]

2025 Mar 31:e0016825. PMID: 40162788
Res Sq

2026 May 20.

Solvente y solubilidad

In Vitro:

DMSO : 5 mg/mL (16.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3586 mL

16.7932 mL

33.5864 mL

5 mM

0.6717 mL

3.3586 mL

6.7173 mL

10 mM

0.3359 mL

1.6793 mL

3.3586 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Mathieu Bourdenx, et al. Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome.Cell. 2021 May 13;184(10):2696-2714.e25. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3586 mL	16.7932 mL	33.5864 mL	83.9659 mL
	5 mM	0.6717 mL	3.3586 mL	6.7173 mL	16.7932 mL
	10 mM	0.3359 mL	1.6793 mL	3.3586 mL	8.3966 mL
	15 mM	0.2239 mL	1.1195 mL	2.2391 mL	5.5977 mL

Keywords

CA77.1

8 Cited Publications

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CA77.1

Autophagy Compound Library

Anti-Alzheimer's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Targeted Diversity Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) CA77.1

Actividad biológica

Chemical Information

Publications (8)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

CA77.1

Autophagy Compound Library

Anti-Alzheimer's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Targeted Diversity Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) CA77.1

Actividad biológica

Chemical Information

Publications (8)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

CA77.1 Related Classifications

HY-134923 Related Classifications