Cyanine5 DBCO hexafluorophosphate
Based on 3 publication(s) in Google Scholar
Cyanine5 DBCO (DBCO-Cy5) hexafluorophosphate is a low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO hexafluorophosphate has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells). Cyanine5 DBCO hexafluorophosphate can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm)
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- Pureza: 98.12%
- No. CAS: 2360411-64-7
- Fòrmula: C53H59F6N4O2P
- Peso molecular:929.03
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Almacenamiento:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cyanine5 DBCO hexafluorophosphate
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Customer Validation & Images
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Cell Imaging/Staining
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Flow Cytometry
Actividad biológica
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1][2].
1. Solution preparation:
1.1 Preparation of storage solution
Solvent: DMSO.
Concentration: 20 mM is recommended.
1.2 Preparation of working solution
Dilute to 20 μM with PBS or serum-free medium (optimized according to the experiment).
(Note: The working solution should be prepared and used immediately, and should be protected from light.)
2. Cell labeling (A549 cells for example):
2.1. Seed cells onto 35-mm glass-bottom dishes at a density of 3×104 (varies by cell) in 2 mL of growth media.
2.2. Add Ac4ManNAz (50 μM, final concentration), incubate for 3 days (generate azide groups on the surface of cells).
2.3. Wash cells twice with DPBS (pH 7.4).
2.4. Incubate cells with Cyanine5 DBCO (20 μM, final concentration) for 1 h at 37°C.
2.5. Rinse cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combined fixative for 15 min at 25°C.
2.6. Wash cells twice with DPBS (pH 7.4) and stain cells with DAPI to label nuclei.
2.7. Measure Cyanine5 DBCO fluorescence (Ex=635 nm, Em=650-700 nm) using a confocal laser scanning microscope.
Note:
1) Artificially introduced azide groups on the cell surface can be targeted by Cyanine5 DBCO for more than 3 days after treatment with Ac4ManNAz.
2) Degree of Cyanine5 DBCO labeling is in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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No. CAS 2360411-64-7
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Appearance Solid
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Peso molecular 929.03
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Fòrmula C53H59F6N4O2P
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Color Dark purple to black
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SMILES
O=C(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C\2(C)C)C=CC=C3)C(C)(C)C4=C1C=CC=C4)NCCCCCC(N5C6=C(C#CC7=C(C5)C=CC=C7)C=CC=C6)=O.[F-][P+5]([F-])([F-])([F-])([F-])[F-]
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Synonyms
DBCO-Cy5 hexafluorophosphate; Cyanine5 dibenzocyclooctyne hexafluorophosphate
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
Bioengineered hybrid dual-targeting nanoparticles reprogram the tumour microenvironment for deep glioblastoma photodynamic therapy. [Abstract]2025 Aug 18;16(1):7672. PMID: 40826003 -
Adv Sci (Weinh)
A Spatially Distributed Microneedle System for Bioorthogonal T Cell-Guided Cancer Therapy. [Abstract]2025 Apr;12(13):e2416841. PMID: 39921874
Cyanine5 DBCO hexafluorophosphate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(13):e2416841. [Abstract]
Representative CLSM images of 4T1 cells stained with Cyanine5 DBCO hexafluorophosphate (DBCO‐Cy5) for 30 min after incubation with IP10‐Az@MNs. Scale bar = 20 µm.
Cyanine5 DBCO hexafluorophosphate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(13):e2416841. [Abstract]
FSC analysis of 4T1 cells stained with Cyanine5 DBCO hexafluorophosphate (DBCO‐Cy5) for 30 min after incubation with Ip10‐Az@MNs.
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Solvente y solubilidad
DMSO : 125 mg/mL (134.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kang SW, et al. Cell labeling and tracking method without distorted signals by phagocytosis of macrophages. Theranostics. 2014 Feb 12;4(4):420-31. [Content Brief]
[2]. Zhu L, et al. Coupling Aptamer-based Protein Tagging with Metabolic Glycan Labeling for In Situ Visualization and Biological Function Study of Exosomal Protein-Specific Glycosylation. Angew Chem Int Ed Engl. 2021 Aug 9;60(33):18111-18115. [Content Brief]
[3]. Song S, et al. In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjug Chem. 2020 May 20;31(5):1562-1574. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0764 mL | 5.3820 mL | 10.7639 mL | 26.9098 mL |
| 5 mM | 0.2153 mL | 1.0764 mL | 2.1528 mL | 5.3820 mL | |
| 10 mM | 0.1076 mL | 0.5382 mL | 1.0764 mL | 2.6910 mL | |
| 15 mM | 0.0718 mL | 0.3588 mL | 0.7176 mL | 1.7940 mL | |
| 20 mM | 0.0538 mL | 0.2691 mL | 0.5382 mL | 1.3455 mL | |
| 25 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
| 30 mM | 0.0359 mL | 0.1794 mL | 0.3588 mL | 0.8970 mL | |
| 40 mM | 0.0269 mL | 0.1345 mL | 0.2691 mL | 0.6727 mL | |
| 50 mM | 0.0215 mL | 0.1076 mL | 0.2153 mL | 0.5382 mL | |
| 60 mM | 0.0179 mL | 0.0897 mL | 0.1794 mL | 0.4485 mL | |
| 80 mM | 0.0135 mL | 0.0673 mL | 0.1345 mL | 0.3364 mL | |
| 100 mM | 0.0108 mL | 0.0538 mL | 0.1076 mL | 0.2691 mL |
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