DIZ-3
DIZ-3 is a selective multimeric G4 ligand based on a G4-ligand-dimerizing strategy. DIZ-3 intercalates into the G4-G4 interface, stabilizing the higher-order structure. DIZ-3 induces cell cycle arrest and apoptosis, and thus inhibits cell proliferation in alternative lengthening of telomere (ALT) cancer cells.
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- No. CAS: 2675490-72-7
- Fòrmula: C46H44F2N8
- Peso molecular:746.89
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BJ | IC50 |
29.3 μM
Compound: DIZ-3
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Cytotoxicity against human BJ cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
Cytotoxicity against human BJ cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
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[PMID: 31759730] |
| U2OS | IC50 |
2.1 μM
Compound: DIZ-3
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Cytotoxicity against human U2OS cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
Cytotoxicity against human U2OS cells assessed as reduction in cell viability measured after 24 hrs by CCK8 assay
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[PMID: 31759730] |
In Vitro
DIZ-3 (0-40 μM; 24 hours) inhibits the proliferation in an ALT cancer cell line[1].
DIZ-3 (0.6-2.5 μM; 24 hours) induces U2OS cell cycle arrest and Apoptosis in ALT cancer cells[1].
DIZ-3 (0.12, 0.25, 0.5 μM; 7 days) causes colony formation and would healing assays carried out in U2OS cells[1].
DIZ-3 (0.12, 0.25, 0.5 μM; 24 h) significantly inhibits migration of U2OS cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human bone osteosarcoma U2OS cells, normal BJ fibroblasts
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Concentration:0-40 μM
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Incubation Time:24 hours
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Result:Caused a significant dose-dependent cytotoxic effect on U2OS cancer cells with an IC50 of 2.1 µM.
Induced much weaker growth inhibition on normal BJ fibroblasts with an IC50 of 29.3 µM.
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Cell Line:U2OS cells
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Concentration:0.6, 1.2, 2.5 μM
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Incubation Time:24 hours
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Result:Induced significant apoptosis in U2OS cells (the percentage of apoptotic cells increased from 10.1% to 24.9%).
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Cell Line:U2OS cells
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Concentration:0.6, 1.2, 2.5 μM
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Incubation Time:24 hours
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Result:Induced the apparent accumulation of cells in the S phase (increasing from 24.0% to 32.2%) in a dose-dependent manner.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- Protocol for Cell Cycle
- CCK-8/WST-8 Cell Proliferation Assay
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Ki-67 Immunostaining Proliferation Assay
- Flow cytometric DNA-content cell-cycle staining
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- Hepatotoxicity Study
- MTT Cell Proliferation Assay
Chemical Information
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No. CAS 2675490-72-7
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Peso molecular 746.89
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Fòrmula C46H44F2N8
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SMILES
CN1CCN(CC1)C2=CC=C(C=C2)C3=C(NC(C4=CC=C(C=C4)C5=NC(C6=CC=C(C=C6)F)=C(N5)C7=CC=C(C=C7)N8CCN(CC8)C)=N3)C9=CC=C(C=C9)F
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DIZ-3 Related Classifications
HY-146812 Related Classifications
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